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Our laboratory in 2002 to carry out molecular breeding of rice, maize DNA fragment into Rice Embryo mediated by ion beam technology, the performance of a group of mutant corn Characters multiple generations of selection, the trait has been basically stable. Selection of four kinds of rice lines with significantly corn traits (aerial roots, purple leaf sheath) as experimental material No. :08-7-114-11-2-2 ,08-7-131-1-1, 08 -7 (2) -131-5-2,08-7 (2) -135-2-1-2-2. AFLP and polymorphism screening analysis of these four kinds of Variants genome through bioinformatics methods for the screening of the target band sequence homology alignment and function prediction and analysis. 64 pairs of selective amplification primers filter the results show that: (1) individual genomic DNA samples (including corn and rice control) amplified bands total number of between 864-980 bar, an average of 942. Corn material amplified bands at least, only 864 the the rice materials than corn material amplified bands were in more than 931. Four kinds of the rice mutant strains average 953 amplified bands. (2) rice lines 08-7-114-11-2-2,08-7-131-1-1,08-7 (2) -131-5-2,08-7 (2) -135 - 2-1-2-2 rice control compared to the increase in the number of bands were 33,34,27 and 29, reducing the number of bands were 45,40,25 and 38.4 kinds of strips of material increased with the total number of bands to 123, reducing the strip with the number 142, the increase and decrease the total number of similar. (3) rice lines 08-7-114-11-2-2,08-7-131-1-1,08-7 (2) -131-5-2,08-7 (2) -135 - 2-1-2-2 compared with rice control, differences in rates of respectively 8.2%, 7.5%, 5.3% and 6.8%, the average difference was 6.9%; various primers amplified material differences Article with a number between 0-8, 1-4 differences with the majority of the difference in rates between 0-50.0% between 5.0-25.0%. (4) rice lines 08-7-114-11-2-2,08-7-131-1-1,08-7 (2) -131-5-2,08-7 (2) -135 - 2-1-2-2 compared with corn control, similar to the rate were 36.7%, 35.1%, 33.6% and 34.7%, respectively;, were higher than the similar rate of 30.9% between rice control and corn control. (5) Variation material 08-7-114-11-2-2,08-7-131-1-1,08-7 (2) -131-5-2,08-7 (2) -135-2 -1-2-2 respectively detected destination strip 11,9,8 and 9 the entry strip. This suggests there may be the corn DNA mediated by the ion beam into the rice genome. Recovery of the objective DNA fragment was cloned and sequenced to obtain the sequence information of the 10 target fragments. NCBI database BLAST homology alignment, the sequence of each target fragment were retrieved one or more high match rate of homologous sequences. P2M3-114-106, P4M4-131-227 the P5M3-131-349 and P5M3-131 (2) -348 entries fragment sequence database source sequence 100% match. 6 of the fragment sequence of the 10 entries, and rice nuclear genome sequence highly homologous; 2 fragment of the entry sequence P5M3-131-348 the P5M3-131 (2) -348 and rice chloroplast genome sequences are highly homologous; 2 entry fragment sequences P1M8-135 (2) -304 P5M3-131-349 and Maize mRNA sequence height matching the former matches the concordance rate was 98% / 97% (two-stage matching), which concordance rate was 100%, which The two purposes fragments may be imported maize DNA fragments. Protein in the NCBI database and EBI database retrieval analytical tools two maize genomic DNA homologous sequences the P1M8-135 (2) -304 and P5M3-131-349 were analyzed, the results show that, P1M8-135 (2) -304 The homologous DNA sequence matched the protein corn hydroxyacyl glutathione hydrolase, the hydrolytic enzyme having a hydrolyzable chemically Antioxidant and detoxification function the P1M8-135 (2) -304 objective gene encoding the synthesis of a protein product hydroxyacyl glutathione hydrolase same or a similar function may have; the P5M3-131-349 DNA sequence to match the protein is maize cysteine ??disulfide endonuclease / ubiquitin protein, mainly involved in protein metabolism. The catalytic processes, are also involved in RNA splicing, P5M3-131-349 object of genes encoding the synthesis of a protein product may have the same enzyme with cysteine ??disulfide or similar function. Which presumably maize DNA mediated effect of the ion beam into the rice genome and expression of the corresponding protein product, and Variant Strains sounding as some corn traits. However, the specific relationship between these proteins function and variability of traits is unclear and needs further study.
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