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Isolation of Antiangiogenesis Peptide from Coina Intestinalis (PCI) and Study of Its Antiangiogenesis Activity

Author: DongPingYuan
Tutor: LinXiuKun
School: Graduate School , Chinese Academy of Sciences ( Institute of Oceanography )
Course: Marine biology
Keywords: Ciona intestinalis Polypeptide Antiangiogenic
CLC: R96
Type: Master's thesis
Year: 2007
Downloads: 206
Quote: 0
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Abstract


Angiogenesis is a must to tumor growth , development , and transfer has a close relationship with the incidence of solid tumors , and inhibition of tumor angiogenesis with high specificity , efficacy , and easy to produce drug resistance , and low toxicity characteristics therefore inhibit tumor angiogenesis is expected to be a breakthrough in the treatment of cancer , the main tumor biological treatment study of antiangiogenic nearly a decade of research has become a hot spot . The glass sea squirt (Coina intestinalis) is a sexual mesh , The Ciona intestinalis Branch, because of its unique position on the evolutionary often as materials to study the evolution of neural development , immune system . Isolated and found a variety of anti-tumor activity of the polypeptide , but other species of sea squirt Ciona intestinalis Purification and activity of anti-angiogenic active peptides have not been reported . This article uses a variety of means of separation and purification using active tracking method for the first time from Ciona intestinalis isolated polypeptide having the role of anti - angiogenesis , and do a preliminary study of its anti-angiogenic activity . This study uses cold acetone fractional precipitation , ultrafiltration, molecular weight cutoff of less than 5 kDa protein , and then separating means by SephadexG25, Superdex75 column chromatography , μRPC C2/C18 reverse phase column chromatography, etc. , using the activity tracking methods from glass ascidians The isolated and purified anti-angiogenic polypeptide PCI, calculated according to the retention time in the molecular weight of 1.8 kDa (Coina intestinalis) . MTT assay showed that human umbilical vein endothelial cells (HUVEC) strongly inhibited , IC50 7.5μg/ml , and a variety of direct inhibition of tumor cell . Zebrafish embryos in vivo experiments further show that the role of PCI 40μg/ml concentration 12h zebrafish embryonic angiogenesis was significantly inhibited intestinal vein vascular length is 30% of the normal group , the normal group, the zebrafish embryo angiogenesis was 45%.

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