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Study on the Regulatory Role of cPTEN in C.elegans Aging by Using RNAi

Author: CaoBo
Tutor: FuXueQi
School: Jilin University
Course: Microbial and Biochemical Pharmacy
Keywords: Nematodes cPTEN RNA interference Senescence
CLC: R96
Type: Master's thesis
Year: 2007
Downloads: 163
Quote: 0
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Abstract


PTEN is a tumor suppressor factor having the activity of the dual specificity protein phosphatases (dual specificity protein phosphatase) , (3,4,5) phosphatidylinositol triphosphate [ PtdIns (3,4,5 ) P3 of of PIP3 ] of the PIP3 levels of co-regulated in vivo dephosphorylation role of phosphoinositide kinase (PI3K) , thereby regulating the second messenger PIP3 - dependent signaling pathway . Caenorhabditis elegans (C.elegans) PTEN the congeners cPTEN by daf-18 gene encoding insulin-like pathway (insulin-like pathway) play an important role . In the present study , we have used RNA interference (RNA interference, RNAi) technology , interference N2 wild-type nematodes and age-1 mutant TJ1052 the nematode cPTEN protein , through the observation of a variety of phenotypic differences , and SOD activity , MDA content and fat accumulation indicators associated with aging changes proved regulatory role cPTEN online insects aging process , provides a theoretical basis for further study of the mechanism of PTEN .

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