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Characteristics of the Gene Expression Profile of Ovarian Clear Cell Carcinoma and Validation of Some Related Important Genes

Author: ZuoZuo
Tutor: WanXiaoPing
School: Shanghai Jiaotong University
Course: Obstetrics and Gynaecology
Keywords: cDNA microarray Gene expression profiling Ovarian clear cell carcinoma Annexin IV Human tissue kallikrein 10
CLC: R737.31
Type: Master's thesis
Year: 2007
Downloads: 113
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Abstract


To establish the purpose of the first part of the gene expression profiles of ovarian clear cell carcinoma : using cDNA microarray technology to establish the gene expression profiles of ovarian clear cell carcinoma , and to explore the molecular mechanisms of poor prognosis in ovarian clear cell carcinoma . Method : one-step eight cases of ovarian clear cell carcinoma and 8 cases of ovarian serous adenocarcinoma total RNA was extracted , reverse transcribed cDNA probes were labeled with Cy3, Cy5 clear cell carcinoma and serous adenocarcinoma . An equal amount of two probes mixed with containing over 14,000 human gene expression microarray hybridization . Jingxi films, scanning , image processing and analysis of the gene expression profile of ovarian clear cell carcinoma and serous adenocarcinoma . Results : ovarian clear cell carcinoma and ovarian serous adenocarcinoma gene expression profile comparison , a total of 82 genes more than 2 -fold difference , which is raised and lowered each of 25 and 57 , can be divided in accordance with the gene functional classification relates to tumor The proliferating transfer multidrug resistance , signal transduction , hormone receptor-related gene known functional genes and some unknown genes . Conclusion : The gene chip can be used for the screening of the gene expression profiles of ovarian clear cell carcinoma , and in-depth study of ovarian clear cell carcinoma of molecular biological characteristics provide important theoretical clues . The second part of annexin IV and human tissue kallikrein 10 specific expression in ovarian clear cell carcinoma Objective: To verify that the 10 ovarian clear cell carcinoma tissue specificity of annexin IV and human tissue kallikrein expressed. Methods: Using quantitative PCR , and immunohistochemistry annexin IV ovarian clear cell carcinoma and human tissue kallikrein 10 expression were detected . Results: The experiment proved that annexin IV ovarian clear cell carcinoma compared with serous carcinoma organizations exist upregulation of human tissue kallikrein 10 serous carcinoma tissue exist downregulated in ovarian clear cell carcinoma than . Conclusion: Annexin IV and human tissue kallikrein enzyme 10 may become a specific tumor marker for ovarian clear cell carcinoma provides a molecular basis as well as a new target for cancer gene therapy .

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Ovarian tumors
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