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Isolation of CD133～+ Cells from Human Renal Cell Carcinoma by Magnetic Cell Sorting System

Author: WuGongZuo
Tutor: YueZhongZuo
School: Lanzhou University
Course: Surgery
Keywords: Kidney Cancer CD133 Cancer stem cells Immunomagnetic beads Gene chip
CLC: R737.11
Type: Master's thesis
Year: 2007
Downloads: 178
Quote: 0
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Abstract


The aim of the present experiment by analyzing CD133 ~ cells in the tumor tissues of patients with renal cell carcinoma , renal cell carcinoma is established CD133 ~ cell separation and purification methods to explore the relationship between of CD133 ~ cell renal cell carcinoma development . The method detected 30 cases of renal cell carcinoma in CD133 ~ cell expression by flow cytometry , and Isolation of purified tumor CD133 ~ cells using gene chip technology to detect CD133 ~ cells and CD133 ~ - cells differences between the gene expression profiles . Results 30 cases of renal cell carcinoma of CD133 cells positive rate average (13.08 ± 1.39)%, with the pathological grade , clinical stage . The immunomagnetic beads isolated and purified CD133 to cell purity (85 ± 2.4)%, cell activity is not affected . Gene chip technology detects CD133 ~ cell group and CD133 ~ - cells compared to eight regulated genes , down 23 , the gene ABCG2 cells between the expression of CD133 ~ cells and CD133 ~ - a significant difference . Conclusion renal carcinoma CD133 ~ cell percentage (13.08 ± 1.39)%. The immunomagnetic beads kidney cancer stem cell research has broad application prospects. Gene BMP2K product CD44 , of IGF1, TDGF1 , of ABCG2 expression in the cell membrane , and the difference is obvious , as further study of kidney cancer stem cells markers .

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Urinary tumors > Kidney,renal pelvis tumor
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