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Bone Marrow CD34+Cells for Prevention of the Complicationsof Vascular Prosthesis Replacement

Author: GaoYong
Tutor: YuZhenHai
School: Shandong University
Course: General Surgery
Keywords: Artificial blood vessels Marrow Endothelialization Patency rate
CLC: R654.3
Type: Master's thesis
Year: 2007
Downloads: 31
Quote: 1
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Abstract


Objective: To investigate bone marrow CD34 cells to grow artificial blood vessels in common ePTFE (expanded polytera fluoroethylene ePTFE) and Dacron artificial vascular endothelial degree and in the long-term patency rates. Method: extraction of canine bone marrow gradient centrifugation to extract the mononuclear cells, and then by the the immunomagnetic beads isolated and purified CD34 cells, were planted in the ePTFE and polyester artificial blood vessels, the dogs were divided into ePTFE vascular experimental group and of polyester vascular experimental group and ePTFE the control group and the the polyester control group, in each group were the same kind of artificial blood vessels implanted in the inferior vena cava and the abdominal aorta with a dog, 10,30,60,100 days after surgery to obtain samples, comparing implant group and patency rates after transplantation of the control group; specimens slice immunohistochemical method identified endothelial cells; observed by gross observation and light microscopy and scanning electron microscopy at different times endometrial hyperplasia and endometrial endothelial neointimal thickness; compare artery endothelialization and patency rates after bone marrow CD34 cells to grow artificial blood vessels and the venous system. Results: (1) dog bone marrow cells isolated by immunomagnetic bead system by flow cytometry identified as CD34 cells. CD34 cells were isolated and purified by immunomagnetic bead system trypan blue cell counting board counting the living cells of 2.6 (± 0.3) × 10 7/ml. (2) the artificial blood vessel wall cells layer after the planting of the the immunohistochemistry Kam CD34 cells to endothelial cells. (3) endothelialization of artificial vascular implants artery occlusion rate ePTFE group stricture rate of 25%; the polyester group stricture rate of 25%. Artificial vascular implants the intravenous the ePTFE group of occlusion rate of 12.5%, 37.5% stenosis; the polyester group occlusion rate, narrow rate of 37.5%. The control group was implanted artery occlusion rate of 0, stenosis rate of 100%; implantable venous occlusion rate of 100% of the control group. (4) the light microscope close and complete, ePTFE neointimal vascular cavity surface partial separation after HE staining with HE staining test group polyester neointimal attached cavity surface. The experimental group two artificial vascular neointimal thickness less than the control group, neointimal thickness, the experimental group two vascular neointimal thickness differences. (5) scanning electron microscope: the experimental group 30 days artificial blood vessels gradually reduced the luminal surface of the mouth to the direction of the middle of the artificial blood vessel neointimal endothelial cell density self-consistent; 100 days of artificial vascular cavity surface endometrial endothelial cells arranged in uniform and complete. Neointima of polyester vascular prosthesis coated with a more complete endothelial cell layer, tightly packed cells, walk the line and consistent with the long axis of blood vessels. ePTFE vascular neointimal compared with the same period polyester vascular neointimal incomplete, the lack of a small amount of area cortex, attached to the surface of cellulose and blood cells and the meantime see necrosis and senescence of endothelial cells. The control group artificial vascular intimal surface of the endothelial cell coverage, only to see the arrangement of the fibrous tissue. Conclusions: (1) After the purified CD34 cells after immunomagnetic bead separation of cell number, proliferation ability, the number of cells able to meet 20-30 cm long, 6-8 mm diameter artificial vessel lumen surface of endothelial planting. (2) by immunohistochemistry identification planted artificial blood vessel wall cells to endothelial cells. (3) purified CD34 cells were seeded on ePTFE and polyester artificial blood vessels, are ideal endothelialization and patency rates. (4) the degree of cavity surface-attached ePTFE artificial blood vessels and endothelial better than polyester artificial vascular CD34 cells after planting. (5) the arterial system of bone marrow CD34 cells to grow artificial vascular endothelial degree and patency rates after planting artificial vascular endothelial degree of bone marrow CD34 cells and patency rates better than the venous system.

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CLC: > Medicine, health > Surgery > Of surgery > Cardiovascular and lymphatic system surgery > Vascular
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