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Genetic Stability of the vaccine strain of yellow fever virus

Author: JiaZuo
Tutor: FengDuoJia；DongXiaoMan
School: Beijing Institute of Biological Products
Course: Immunology
Keywords: Yellow fever virus Yellow fever vaccine Yellow fever vaccine 17D strain Tissue culture Genetic stability Sequencing
CLC: R392
Type: Master's thesis
Year: 2007
Downloads: 59
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Abstract

Yellow fever vaccine is highly attenuated live virus vaccines, and their production matrix is ??7 day old chick embryos, has been safely used for over 50 years in the world. In this study, the 17D strain of yellow fever vaccine virus in chicken embryo fibroblasts consecutive passages of passaged virus genetic stability study, explore the tradition for the yellow fever vaccine production the chick embryo Change for chicken embryo fibroblasts , whether the impact of the genetic stability of the vaccine, chicken embryo fibroblasts for use as the matrix of the yellow fever vaccine production research routes provide some basis. Yellow fever vaccine of international standard 99/616 (NCBI number) and of yellow fever vaccine virus seed in this experiment in 2006 (54) 0239 (Tiantan Biological number) as experimental passaged virus seed, both 17D vaccine strain , 99/616 isolated from 17D-204 strains. Two toxic species in chicken embryo fibroblasts adaptability passages, each pass 10 generation, denoted as \1,3,6,9 generation virus to select 99/616-CEF passaged virus and YFVBJ-CEF passaged virus and its nucleotide sequence of the structural gene fragment of C, M, E, were measured. In addition, our goods with the nucleotide sequence of the yellow fever vaccine were measured, the vaccine virus seed is 2006 (54) 0239 culture medium for the 7-day-old live chick embryo. The sequencing results of data analysis consists of three parts: analysis 99/616-CEF passaged virus genetic stability. The sequencing results 99/616-EEF passaged virus published NCBI GENEBANK the nucleotide sequence of the 17D-204 strain of yellow fever vaccine for comparison. Second: the analysis of the passaged virus YFVBJ-CEF genetic stability. Passage virus YFVBJ-CEF with our goods with the yellow fever vaccine sequencing results comparison. : Analysis of the historical background of yellow fever vaccine virus seed. China's commodity 17D-204 with the sequencing of the yellow fever vaccine and NCBI GENEBANK announced 17DD yellow fever vaccine strain nucleotide sequence comparison. Analysis inferred by the experimental results: the yellow fever vaccine virus after passaged chick embryo fibroblasts, and its genetic material occurs adaptive changes. 99/616-CEF passaged virus YFVBJ-CEF passaged virus structural gene fragment C were more active nucleotide sites; structural gene fragment M, E passaged virus in the 99/616-CEF more active, while the no change in BJYFV-CEF passaged virus. Yellow fever vaccine seed from the attenuated vaccine strain of yellow fever virus 17D strain, with 17D-204, 17DD strain difference in the level of the gene may be another offshoot of the 17D-204 vaccine strain.

Genetic Stability of the vaccine strain of yellow fever virus

Abstract

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