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Effect of Collagenase and Its Inhibitors on the Durability of Dentin Bond

Author: ZhengXinYu
Tutor: ChenZuo
School: Zhejiang University
Course: Clinical Stomatology
Keywords: AFM Dentin Collagen fibers Collagenase Collagenase inhibitor Microtensile bond strength
CLC: R781.2
Type: Master's thesis
Year: 2010
Downloads: 24
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Abstract


Dental caries, trauma and other causes defects in clinical practice is very common. In dental composite resin bonding defects, no mercury pollution the ground less tooth structure, beautiful and has good biocompatibility, has been widely used in clinical practice. The special structure of the dentin, contains a lot of organic matter, and composite resin polymerization shrinkage characteristics, it is not easy in the dentine to form a stable bond, especially to maintain the bond of lasting performance. The composite resin and dentin formation of the basic mechanism is the formation of the mixed layer, collagen fibers is a major organic component of the mixed layer, the integrity of the adhesive to maintain a stable, play an important role. Recent studies show that dentin containing the endogenous activity of procollagen / liquefaction of gelatin, this enzymatic activity is caused by the degradation of dentin collagen fibers, will destroy the integrity of the mixed layer is not conducive to a lasting performance on dentin bonding. , thus affecting the long-term clinical effects of bonded restorations. After the etching dentin surface composition and bonding monomer composite resin composition formed by the bonding interface is the weakest parts of dentin the the dentin surface bonding interface appropriate treatment is the key to the success of dentin repair . This study, using an atomic force microscope (AFM) observation dentin by acid etching to form the morphology of the organic layer (collagen fibers); On this basis, the use of exogenous collagenase teeth in situ AFM observation essentially of collagen fibers enzymatic test,-analog dentin endogenous collagenolytic activity, and explore the response characteristics; study different exogenous collagenase inhibitor on the inhibition of the enzymatic reaction. Secondly, people teeth vitro study, the use of micro-tensile bond strength test (μTBS), scanning electron microscopy (SEM), research dentin bonding the of exogenous collagenase inhibitor on dentin bond the influence of the strength and adhesion persistence inferred to inhibit the dentin endogenous enzyme activity in the actual bonding on the bond, in order to explore the factors and mechanisms affect dentin persistence. Experimental purposes and methods: 1, the use of AFM of dentin collagen fiber imaging research. Preparation for AFM observation of the dentin samples through appropriate treatment, exposure to the dentin surface of the collagen fiber network, the use of AFM for scanning, and morphology of collagen fibers to form a clear image. Observation the dentin organic matter (collagen fibers) surface morphology, and provide a research platform for subsequent experiments. 2, the use of the AFM images of the dentine collagen fibers in collagenase degradation process. The dentin samples placed in the the AFM liquid pool, by etching after injection formulated collagenase I, collagenase II mixed solution, the use of AFM situ in the liquid environment of continuous scan observation collagenase and dentin organic matter (mainly collagen fibers) AFM scanning topography variation of enzymatic reaction process, record the experimental process, explore the microscopic changes in the characteristics of of dentine collagen fibers in collagenase. 3, AFM observation of different collagenase inhibitor on the inhibition of the reaction of collagenase and collagen fibers. Chlorhexidine, captopril as collagenase inhibitor using AFM observed inhibition of the reaction process of collagenase II collagen fibers. According to the test results to infer the effect of collagenase inhibitors inhibit collagenase enzymatic reaction. 4, to the human third molars vitro study, study dentin collagenase inhibitor lasting impact on the bond application. Vitro teeth, removing the coronal tooth enamel, and the preparation of the standard dentin adhesive surface, after etching, before bonding application of collagenase inhibitors (gluconate chlorhexidine), to complete the bonding, composite resin layering Preparation microtensile specimen, and the aging test. Instantly μTBS using μTBS test measures by μTBS after 6 months of aging, and the use of SEM observation of changes in the bonding interface components, comparative studies, evaluation bonding collagenase inhibitor effects, and infer the corresponding mechanism. Results: 1, establish the use of the AFM scan imaging method of dentin collagen fibers, obtain a clear collagen fibers AFM scan image. The dentin collagen fiber reticular characteristic periodic stripes structure is visible using the AFM software analysis, the periodic structure of the collagen fiber width of about 67 nm. 2, the use of AFM in situ observed in the the dentin organic matter (collagen fibers) in enzymatic process collagenase Ⅰ, Ⅱ role. Under the action of collagenase, dentin collagen fiber network is increasingly evident over time change, the process post-fiber web thinning, eventually exposing the mineralized dentin base combined state of the collagen fibers. By this method, can be observed at the bottom of the fine structure of the dentin group. 3, collagenase Ⅱ effective degradation of the dentin organic matter (collagen fibers). Dentin surface morphology of the treatment solution containing collagenase II changes significantly, and a large number of collagen fibers was pre-processed into a network, the process of collagen fibers was significantly reduced after 6 hours, and exposing a large number of the dentin mineral base image. 4, chlorhexidine, captopril can inhibit the activity of collagenase Ⅱ. Collagenase II treatment solution of chlorine-containing chlorhexidine the collagenase II with captopril treatment solution with the dentin organic matter (collagen fibers) role the dentin organic matter (collagen fibers) morphology before and after the experiment did not happen apparent changes. Dentin collagenase inhibitor (gluconate chlorhexidine) can enhance the persistence of the dentin. Microtensile specimens of the control group prepared without collagenase inhibitor, after 6 months of water storage aging, the greater the extent of the decline in bond strength, there is a statistically significant difference (p lt; 0.05). Micro tensile specimen fracture surface SEM results show that the type of fracture of the experimental group and the control group, the difference after 6 months of water storage aging. Application of collagenase inhibitor in the control group in the cleavage site located in the bottom of the mixed layer, and significantly reduced the amount of collagen fibers. Conclusion: 1, AFM is a real-time study the dentin collagen fiber effective method. Real-time observation of the degradation of of dentine collagen fibers in collagenase. 2, collagenase II can effectively degrade the dentin organic matter (collagen fibers), the outer endogenous collagenase inhibitor (chlorhexidine, captopril) can inhibit the activity of collagenase Ⅱ. 3, application collagenase inhibitor (gluconate chlorhexidine) can improve the persistence of the dentin.

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CLC: > Medicine, health > Oral Sciences > Oral medicine > Caries in teeth diseases
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