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AFM Observation of Collapse and Expansion in Collagen Fibrils Network of Dentin Treated with Different Echants/primers

Author: SunJingQing
Tutor: GuXinHua
School: Zhejiang University
Course: Prosthodontics
Keywords: AFM Dentin Etchants Collagen fibers Demineralization degree Subside
CLC: R781.2
Type: Master's thesis
Year: 2011
Downloads: 24
Quote: 0
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Abstract


Objective: This issue through atomic force microscopy (Atomic Force Microscopy, AFM) to observe and measure the air before and after treatment in solution and demineralized dentin collagen network height of the pre-treatment of the different bonding agents / etchants etching of dentin the extent to that of the demineralized dentin get intuitive awareness, provide clinical guidance for the selection of a suitable binder . MATERIALS AND METHODS: collection of adult orthodontic unplug the health of caries-free defects third molars , occlusal to cut about 2mm dentin flakes , followed by polishing shock dentin samples prepared for AFM observation 20 . Randomly divided into four groups of five . The dental film side covered with Masking the other side is in strict accordance with the use of pre-treatment of the various adhesive systems bonded agent or acid etching agent handling ready, dentin plane , and the aqueous solution was observed in the AFM air interface , The resulting diagram using the AFM analysis software for testing , final data analysis using SPSS16.0 . Results: either in the air or observed in solution , a height difference of Clearfil SE Bond and iBond - processed than 37 % phosphoric acid and SuperBond CB are small ( p LT ; 0.05 ) . Was significantly less than 37% phosphoric acid ( p LT ; 0.05 ) after treatment in the air , SuperBond CB height difference . In solution , the difference in height of the iBond after treatment was significantly higher than that of Clearfil SE Bond (p lt; 0.05). Dentin specimens after etching agent / treatment agent in each group , the difference in height in the air are significantly greater than the solution of the height difference (p lt; 0.05) Conclusion : the pre-treatment of the different bonding agent / etching agent on the teeth of this degree of quality etching ; degree of dentin demineralization total etch systems etching treatment , the degree of dehydration collapse of the collagen fiber network is significantly higher than the self-etching systems ; treatment either etch system , demineralization dentin collagen fibers present in the solution in the air over the apparent collapse .

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CLC: > Medicine, health > Oral Sciences > Oral medicine > Caries in teeth diseases
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