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Inhibiting Effects of Serms Alone and the Combination with Cisplatin on the Human Tongue Carcinoma Cell Line Tca8113

Author: ZhouJie
Tutor: SunGuoHong
School: Huazhong University of Science and Technology
Course: Clinical Stomatology
Keywords: Tongue cancer ER-α ER-β Tamoxifen Cisplatin
CLC: R739.8
Type: Master's thesis
Year: 2010
Downloads: 22
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Abstract


Objective: tongue squamous cell carcinoma is one of the common malignant tumors of the oral and maxillofacial region. Treatment methods are mainly based on surgery, before or after surgery using radiotherapy and chemotherapy, the 5-year survival rate of approximately 48.0% to 70.9%, the cause of treatment failure is mainly local and regional recurrence. Contribute to the reduction of the lesion, preoperative chemotherapy and postoperative chemotherapy can help prevent the spread of lesions. Tamoxifen (tamoxifen, TAM) is a selective estrogen receptor modulators (selective estrogen receptor modulators, SERMs), as the endocrine treatment of breast cancer, has been applied for many years in clinical practice. Cisplatin (cisplatin, DPP) is a class of broad-spectrum chemotherapy drugs, chemotherapy for a variety of solid tumors. The subject for the first time the combined effects of tamoxifen and cisplatin in human tongue squamous cell carcinoma of Tca8113 and its effects with effects of the two drugs are compared, to study the efficacy of the combination of the two is superior to single-agent and its mechanism of action be discussed. Methods: In vitro cultured human tongue cancer cell line Tca8113, using Immunocytochemical Detection of estrogen receptor (estrogen receptor, ER) Tca8113 cells exist expression. The morphological changes of the cells using an inverted microscope. TAM and DDP alone or in combination with medication role in Tca8113 cells, MTT colorimetric determination of cell growth inhibition. Results: In human tongue squamous cell carcinoma Tca8113 cell lines were found in the expression of ER-α and ER-β, ER-β expression was significantly higher than the expression of ER-α. TAM group, DDP group and the two-drug combination group were found by cell morphology observed visible typical morphological changes in the cells after treatment, while no such morphological changes in the control group. MTT colorimetric analysis showed that the TAM group, DDP group and the two drugs combined group could inhibit the proliferation of tumor cells; TAM group, DDP group and the two drugs combined group according to the formula: inhibition rate (IR) = (1 experimental group OD value / control OD value) × 100% to calculate the inhibition rate of different concentrations of each group of drug. TAM group 1μmol / L 2μmol / L 5μmol / L, 10μmol / L 24 h inhibition rate were 2.83%, 13.38%, 46.67%, 64.76%; the DDP group in 0.1μg/ml, 1μg/ml, 5μg / ml, 24 h 10μg/ml inhibition rate were 4.69%, 10.27%, 27.19%, 51.37%, with significant inhibition of proliferation (P lt; 0.05). Both human tongue squamous cell carcinoma Tca8113 inhibition with time and concentration are closely linked (P lt; 0.05). Combined with TAM (5μmol / L) and DDP (5μg/ml) 24 h after medication, compared with 5 μmol / L, 10μmol / l TAM group and 5μg/ml, 10μg/ml DDP group, the two-drug combination of cell proliferation The inhibition rate was significantly higher than the two single-drug group, a statistically significant difference (P lt; 0.05). Conclusion: Tca8113 human tongue squamous cell carcinoma cell lines can be detected expression of ER-α and ER-β and ER-β than ER-α expression, which means that both the human tongue the occurrence plays a different role in the development process. Tamoxifen and cisplatin can tongue squamous cell carcinoma cell line Tca8113 proliferation in vitro inhibition of joint use of the two drugs, the inhibition of cell proliferation and cytotoxicity have strengthened. Its role and direct relationship between drug concentration and effect time. 3 resulting in inhibition of cell proliferation and apoptosis by inhibition of protein kinase C (protein kinase C, PKC) vitality as well as to promote the secretion of transforming growth factor β1 (transforming growth factorβ1, TGF-B1), which is believed may be tamoxifen one of the mechanisms of cisplatin and combination of apoptosis in Tca8113 cells in vitro induced human tongue squamous cell carcinoma. Two-drug combination group apoptosis was higher than the two single-drug group may be due to tamoxifen improves the sensitivity of cells to cisplatin, resulting in apoptosis enhanced role.

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CLC: > Medicine, health > Oncology > Oral cavity, maxillofacial tumors
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