Dissertation > Excellent graduate degree dissertation topics show

Effect of Endothelin-1 on Cyclooxygenase-2 Expression in Human Hormone Refractory Prostate Cancer Cells

Author: SuZuo
Tutor: JiaRuiPeng
School: Nanjing Medical University
Course: Surgery
Keywords: endothelin-1 cyclooxygenase 2 prostate cancer gene expression
CLC: R737.25
Type: Master's thesis
Year: 2009
Downloads: 21
Quote: 0
Read: Download Dissertation

Abstract


Objective: To explore the effects and possible mechanism of recombinant human endothelin-1(ET-1) on cyclooxygenase-2 (COX-2) expression in human androgen independent refractory prostate cancer (AIPC) cells (PC-3 cells).Methods: PC-3 cells were treated with 100nmol/L ET-1 for indicated hours(3, 6, 9, 12, 24h)and indicated concentration (0.1, 1, 10, 100 nmol/L) for 24 h. Besides, 100 nmol/L ET-1 was used to treat PC-3 cells alone or in combination with endothelin A receptor(ETAR)antagonist BQ-123(1μmol/L), endothelin B receptor(ETBR)antagonist BQ788 (1μmol/L), MAPK/extracellular signalregulated kinase kinase (MEK)-selective inhibitor, PD98059 (10μmol/L), p38 mitogen activated protein kinase (MAPK) antagonist SB203580 (5μmol/L) and epidermal growth factor receptor (EGFR) antagonist AG1478(0.1μmol/L)for 24h. COX-2 mRNA and protein expression in PC-3 cells was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis.Results: ET-1 induces a time and dose-dependent increase in mRNA or protein expression of COX-2 in PC-3 cells. BQ123, PD98059, SB203580 and AG1478 could prevent the expression of COX-2 in PC-3 cells (P<0.05) while BQ-788 couldn’t. Conclusion: ET-1 could induce the up-regulation of COX-2 in PC-3 cells. ETAR might be involved in the course. Several signaling pathways, including p42/44 MAPK, p38 MAPK and EGFR have been implicated in the regulation of COX-2 expression.

Related Dissertations

  1. Research on Combinatorial Regulation of Multiple Transcription Factors,Q78
  2. The Expression Survey of BMP Signalling Pathway in the Human Embryonic Tooth Germ,R78
  3. Study on Relationship between Endothelin-1, Insulin Resistance and Acute Ischemic Cerebrovascular Disease,R743.3
  4. Study on Synthesis and Physiological Function of N-carbamoyl-glutamate,R914
  5. Functional Analysis of Two Rice Nitrate Transporter Genes OsNRT1.1a and OsNRT1.1b,S511
  6. Coparative Analyses of Carbon and Nitrogen Metabolism of Flue-cured Tobacco Growing in Henan and Yunnan,S572
  7. Study of Chicken Δ~6 Fatty Acid Desaturase Gene’s Promotor Region Polymorphism and Gene Expression,S831
  8. The Expression of CPV-2 VP2 Gene in SF9 and Development of Indirect Elisa for Serum Antibodys,S852.65
  9. Comparison of Gene Expression Data Cluster Methods and Gene Network Construction for Phytophthora Sojae Genes,S435.651
  10. Functional Identification of Two Tomoto Phosphater Transporter Genes LePT1 and LePT2 in Rice,S511
  11. Analysis for Intron 1 and 2 of Rice Transformation to Promoter Activity,S511
  12. Research on Characteristics of Growth, Development and Material Metabolism of Flue Cured Tobacco Leaves in Huili, Sichuan,S572
  13. Studies on Extracting Mitochondrial DNA and Gene Expressions in Brassica Napus L,S565.4
  14. Functional Characterization of Wheat Na~+ /H~+ Antiporter TaNHX2 and Study of TaNHX2 C- Terminus Domain,S512.1
  15. Identification and Analysis of miRNA and Flower Development Specific Genes in Wheat,S512.1
  16. Molecular Characterization, Expression, and Promoter Methylation Status of the Bovine Synaptonemal Complex Protein 3 Gene,S823
  17. The Deposition Rule and Gene Expression Involved in Yolk Cholesterol in Different Layer Breeds,S831
  18. Study on the mRNA Expression Level and the Polymorphism of Follicle-Stimulating Hormone Receptor Gene in Taihu Pig’s Ovary,S828
  19. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  20. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  21. The Design and Implementation of Bicluster Data Analyzing Software,TP311.52

CLC: > Medicine, health > Oncology > Genitourinary tumors > Male genitalia tumors > Prostate cancer
© 2012 www.DissertationTopic.Net  Mobile