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Curcuma oil injection Objective To observe the different concentrations of the inhibition of human ovarian cancer cell line SKOV3, and to explore the possible role of principles and mechanisms to provide experimental evidence for this medicine clinical application of the treatment of ovarian cancer. The method MTT assay in human ovarian cancer SKOV3, treated with different concentrations (120ug/ml, 240ug/ml, 480ug/ml, 960ug/ml) the Curcuma oil processing cell proliferation calculated cell inhibition rate (%) = (1 - the absorbance value of experimental group / absorbance values ??of the control group)] × 100%. The experiment was repeated three times to take the average of three results. SKOV3 cells morphological changes in the optical microscope before and after treatment were divided into blank control group and the experimental group, the experimental group is divided into four concentrations: 120u g / ml Curcuma oil, 240ug/ml Curcuma oil, 480u g / ml Curcuma oil, 960ug / ml the Curcuma oil, medication after 48 h of incubation the cells were collected, fixed, HE staining. Immunohistochemical method (kit instructions) cells of the control group and the experimental group were detected by both Bcl-2 and Bax protein expression changes. Results 1 the Curcuma oil on the proliferation of human ovarian carcinoma cell line SKOV3 inhibit its intensity is enhanced with increasing time and concentration of drug action was a dose-dependent manner. Curcuma oil apoptosis of SKOV3: blank control group: cells were spindle or polygonal cell membrane and the nucleus full, abundant cytoplasm, pink, magenta, the nucleus center, different nuclear morphology can be dual-core or multi-core phenomenon, clear nucleolus cells thrive, no apoptotic bodies. Experimental group: Curcuma oil for 48h, cell morphology change significantly. Decrease in adherent cells, cell swelling, increased volume, rough edges irregular concentrated cytoplasmic dense granules in the cytoplasm of the cell membrane integrity undermine part karyopyknosis, no obvious apoptotic bodies. Immunohistochemistry to detect groups of Bax and Bcl-2, two protein expression, compared with the control group, the Bax protein expression was significantly increased in the experimental group, the difference was statistically significant (p lt; 0.05), while the Bcl-2 protein expression was significantly reduced, and the difference was statistically significant (p lt; 0.05). Conclusion 1. The Curcuma oil on human ovarian cancer cells SKOV3 has obvious inhibition of proliferation and induction of apoptosis. 2 the Curcuma oil induced ovarian cancer the SKOV3 cell apoptosis in, it may be a certain relationship with downregulation of Bcl-2 expression, and raised the expression of Bax.
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