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Screnning of Gene Fusion in Esophageal Squamous Cell Carcinoma

Author: ZhanTing
Tutor: ZhangShengZhou;WangMingRong
School: Anhui Normal University
Course: Biochemistry and Molecular Biology
Keywords: Esophageal squamous cell carcinoma Genome Walker Chromosomal translocation Fusion gene
CLC: R735.1
Type: Master's thesis
Year: 2010
Downloads: 25
Quote: 0
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Abstract


The pathogenesis of esophageal squamous cell carcinoma has so far failed to obtain clear results , including surgery and new treatment overall survival rates are not high . To solve this problem , this study aims to study the fusion gene in esophageal squamous cell carcinoma . The experiment based on genomic DNA chip ( array - CGH ) technology , possible translocation breakpoints in esophageal squamous cell carcinoma cell lines will shrink to about 70Kb , quantitative PCR that the area is further reduced to less than 600 bp genomic walking technique where is the key to the whole experiment , the use of its available unknown fracture fragment translocation results finally obtained by sequencing . After analysis and verification , the present study found that the t ( 2 ; X ) ( q33, esophageal squamous cell carcinoma ; q26 by ) translocation , ie PLCL1 FGF13 fusion . The results of this study to prove , genome walking technology can effectively achieve the purpose of the discovery of the fusion gene . It break through the traditional the fusion gene research methods , while saving the cost of experiments . Esophageal squamous cell fusion gene has not yet been found integration of PLCL1 and FGF13 , may lead to disorders of the FGF signaling pathway , the chromosomal rearrangements may play a key role in the formation of the tumor , its treatment is a useful reference .

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Esophageal tumors
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