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The purpose of the effects of vitamin C on lung cancer A549 cell proliferation, apoptosis and arsenic trioxide (AS 2 O 3 ), cisplatin (DDP) on the proliferation of lung cancer A549 cells, synergy of apoptosis, further explore vitamin C A549 cell proliferation and apoptosis of possible mechanisms. A549 cells in vitro with different concentrations of vitamin C the DDP, AS 2 the O 3 cisplatin vitamin C, the AS 2 O 3 vitamin C, cell growth curve and clone formation assay cell growth; 400 ug / ml concentration of vitamin C role in lung cancer A549 cells, respectively, in its role 6h, 12h 24h, 48h underwent flow cytometry cell cycle and apoptosis rate and total RNA was extracted and protein, RT-PCR was used to detect the SurvivinmRNA, Caspase-3mRNA, MDR1, MRP1mRNA expression, Western blot detection of Survivin protein expression of caspase-3 protein . Results of the 1. Growth curve: 40ug/ml, 400ug/ml, 4mg/ml vitamin C (1/10 clinical dosage, times the clinical dosage 10 times the clinical dosage) role in lung cancer A549 cells, growth curve, found no significant effect of the 40ug/ml vitamin C on the proliferation of A549 400ug/ml vitamin C significantly inhibited the proliferation of A549 cells. To clinical dosage i.e. 400ug/ml of vitamin C were combined 0.4 ug / ml, 4 ug / ml, 4 0ug/ml (1/10 clinical dosage, the clinical dosage of 1 times, 10 times of clinical dosage) DDP role in A549 cells, rendering growth curve found 400ug/ml of vitamin C combined 0.4 ug / ml of DDP already significantly inhibited the proliferation inhibition rate alone 0.4 ug / ml DDP 400ug/ml vitamin C, there is a significant difference; single 4 ug / ml, and no significant difference when 4 0ug/ml the DDP. 400ug/ml of vitamin C were combined 0.04ug/ml, 0.4 ug / ml, 4 ug / ml, 4 0ug/ml (clinical dosage of 1/100, 1/10 clinical dosage, 1-fold clinical dosage. 10 times the clinical dosage) AS the AS 2 O 3 role in A549 cells, the growth curve found 400ug/ml of vitamin C combined 0.04ug/ml AS 2 O 3 O 3 when already significantly inhibit the proliferation of tumor inhibition rate alone 0.04 ug / ml > 400ug/ml of vitamin C were significantly different; with 4 ug / ml alone AS 2 O 3 no significant difference. Colony formation experiments: 400ug/ml vitamin C under the A549 cell clones significantly reduced, significantly reduce the rate of tumor formation. The the DDP when inhibition rate of single 400ug/ml of vitamin C combined with 0.4 ug / ml with 0.4 ug / ml DDP 400ug/ml of vitamin C were significantly different. 400ug/ml of vitamin C the joint 0.04ug/ml the AS 2 O 3 inhibition rate of the single with 0.04 ug / ml AS 2 O 3 , 400ug/ml of vitamin C, a significant difference. Flow cytometry: of 400ug/ml vitamin C role in A549 cells were role 6h, 12h, 24h, 48h, A549 cells arrest in G0/G1, S phase, and cells wither as the prolonged duration of action The death rate is gradually increased. The flow cytometry results showed that the DDP group and DDP in combination with vitamin C group A549 cells at G0/G1 phase significantly prolonged the A549 cell cycle DDP in combination with vitamin C group was significantly synergistic increase in apoptosis of cells. AS 2 O 3 the Unit and AS 2 O 3 combined with vitamin C group A549 cells were arrested in G0 / G1 period significantly longer A549 cell cycle, and AS 2 O 3 associated with vitamin C group was significantly synergistic increase in apoptosis of cells. : Vitamin C can up-regulate the expression of Caspase-3mRNA 4.RT-PCR detection; the expression of SurvivinmRNA no significant impact; vitamin C did not significantly affect MRP1mRNA expression, but does not affect the AS 2 O raised on the basis of 3 induced apoptosis expression MDR1mRNA. 5. Western blot detection: Vitamin C can up-regulate the expression of Caspase-3 protein; Survivinm protein expression was no significant effect on the conclusions of this experimental in vitro proved based linking vitamin C with the the AS 2 O < sub> 3 , DDP obvious synergy to inhibit proliferation of lung cancer A549 cells and co-promote apoptosis and to reduce the the AS 2 O guarantee efficacy , DDP dosage. Caspase-3 synergistic mechanisms may prolong cell cycle and raised to promote tumor cell apoptosis.
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