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Mouse Model of Gastric Cancer and Changes in Level of Regulatory T Cell (Treg)

Author: XuYiZuo
Tutor: ZhuLiZuo
School: Tianjin Medical University
Course: Surgery
Keywords: Gastric cancer CD4 ~ CD25 ~ Regulatory T cells Flow cytometry T cell subsets
CLC: R735.2
Type: Master's thesis
Year: 2009
Downloads: 47
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Abstract

Objective 3 - methylcholanthrene (3-methylcholanthrene ,3-MCA) and N-methyl-N-nitrosourea (N-methyl-N-nitrosourea, MNU)-induced mouse gastric model. White blood cell count, respectively, at 12 weeks and 20 weeks of mice peripheral blood and spleen cells, CD4 CD8 T cell subsets CD3 and CD4 CD25 regulatory T cells (CD4 CD25 regulatory T cells, Treg) in mouse gastric model building process period level variations. This experiment is a six-week-old, male, BALB / c mice to 3-MCA hanging line method in mice proventriculus hanging containing 3-MCA line knot United MNU gavage in mice induced gastric cancer. Were detected at 12 weeks and 20 weeks of blood and spleen cells white blood cell count, and their T-cell subsets and Treg levels detected by flow cytometry (Flow Cytometries, FCM), gastric cancer development at the same time take the the gastric specimens slice for pathological examination situation. The analysis of the data obtained by statistical methods. 1.12 weeks, 25% of the mice proventriculus parts mucosal thickening and chronic inflammation. 20 weeks, 37.5% of the mice successfully induced murine gastric cancer. Control mice showed no mucosal thickening, chronic inflammation or gastric cancer. Experimental mice at 12 weeks and 20 weeks reduced weight change compared with the control group were statistically significant (P lt; 0.05). 3 experimental groups of mice the spleen leukocyte count was statistically significant (P lt; 0.05) in the 20 weeks increased. Experimental mice peripheral Treg rise in the 20 weeks was statistically significant (P lt; 0.05). Of spleen Treg proportion of 12-week and 20-week rise were statistically significant (P lt; 0.05). Spleen Treg reduction of the absolute value in the 12 weeks to 20 weeks increases were statistically significant (P lt; 0.05). 5. Experimental mice spleen CD3 cells increased in the 20 weeks was statistically significant (P lt; 0.05). Splenic CD4 cells with a statistically significant reduction of 20 weeks (P lt; 0.05). Splenic CD8 cells in a significant reduction of 20 weeks (P lt; 0.05). Splenic CD4 / CD8 ratio increased in the 20 weeks was statistically significant (P lt; 0.05). 6. Absolute value of the experimental mice spleen CD3 cells in the 20 weeks increased with statistical significance (P lt; 0.05). The spleen CD4 cell absolute value in 20 weeks, the increase was statistically significant (P lt; 0.05). The conclusion 1.MCA method combined with MNU the gavage method successfully in mice induced gastric hung. Long-term exposure to carcinogens can lead to weight loss, weight growth recovered slightly in stop carcinogens attacks for some time, but can not return to normal levels. As a result of long-term exposure to carcinogens, leading to tumor can cause weight loss complex. Long-term exposure to carcinogens can cause the reduction of the white blood cell count, white blood cell count level is still not back to normal levels even stop carcinogens attack but spleen presents elevated white blood cell count trends, the reason may be related to the immune system lymphocytes reactive hyperplasia. 4.Treg play a role in the regulation of autoimmune and play an important role in tumor occurrence and development process. Its overexpression prevention inflammation itself cause excessive immune response itself tissue damage, while in the process of tumor formation, inhibiting the body to produce an effective anti-tumor immune response, leading to tumor immune escape. 5.T cell subsets induced early inflammatory response in gastric cancer have increased in the total number of T cells and the CD4 cell level, because high expression of Treg prevent excessive immune cause CD8 cells decreased. Late part tumor immune function is affected in mice led to the decrease in the total number of T cells and CD8 cells induced in gastric because of inflammation or tumor effects, but still result in elevated CD4 cells.

Mouse Model of Gastric Cancer and Changes in Level of Regulatory T Cell (Treg)

Abstract

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