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Objective: To analyze oxalic platinum processing posterity colon cancer LoVo cell line differences in expressed proteins, mechanism of action of oxaliplatin in human colon cancer LoVo cell line from proteomics angle to explore. Method: In accordance with the MTT assay detection of cell proliferation, calculated oxaliplatin median inhibitory concentration. 100ml contains half inhibitory concentration of oxaliplatin medium of LoVo cell lines after 24h the cells were collected for the experimental group, drug-free medium LoVo cell lines after 24h after the cells were collected for the control group. After hydration, isoelectric focusing, balancing, vertical gel electrophoresis groups of total protein were isolated, and then stained with Coomassie blue, scanned two-dimensional gel electrophoresis pattern. Analysis of the gel image, look for two sets of differentially expressed proteins. Differentially expressed proteins identified by mass spectrometry and bioinformatics analysis. Results: the experimental group and the control group, the number of protein spots for 1013 and 1021. Average match the experimental group of 812, 829 control group. Select one of the most significant difference of 10 points MALDI-TOF-MS analysis, successfully identified nine proteins. They were downregulated protein in the experimental group: A1 No. 60S ribosomal protein A2, A4 No. 3 phosphate dehydrogenase, A3 aldo-keto reductase 1-C2, A5 guanylate binding protein-like 1, A6 sign nucleoside diphosphate kinase B; experimental group upregulation of protein: B7 No. β2 microglobulin, B8 Pre-mRNA splicing factors, B9 uneven ribonucleoprotein H3 uracil glycosidase B10. Differences in protein and DNA damage repair, transcriptional modification of pre-mRNA and c-myc gene transcription factor PuF protein translation and post-translational regulation of immune escape mechanism of signal transduction pathways, such as energy metabolism and induce apoptosis related. Conclusion: The role of oxaliplatin colon cancer LoVo cell lines 60S ribosomal proteins and other differences in protein expression; related differences in protein and DNA damage repair mechanisms; prompted oxaliplatin anticancer mechanism may be related to the above mechanisms. Objective: To analyze irinotecan treatment descendants colon cancer LoVo cell line differences in expressed proteins, from proteomics angle to explore the mechanism of action of irinotecan on human colon cancer LoVo cell lines. Methods: In accordance with the MTT assay detection of drug proliferation inhibition, calculated irinotecan, topotecan half inhibitory concentration. 100ml contains half inhibitory concentration irinotecan healthy medium role in the Department of LoVo cells after 24h, 48h and 72h after the cells were collected for experiments B, C, D group, with drug-free medium LoVo cell lines after 24h The cells were collected for the control group A. Hydration, isoelectric focusing, balancing, vertical gel electrophoresis and stained with silver nitrate to obtain a two-dimensional gel electrophoresis pattern, the scanning and analysis, looking for the two sets of differentially expressed proteins. Differentially expressed proteins identified by mass spectrometry and bioinformatics analysis. Results: the experimental group and the control group, the average number of protein spots 1100,1087,1097 and 929, and the average match points to 965,892,920 and 758. Select one of the most significant difference of 17 points for the MALDI-TOF-MS analysis, successfully identified nine proteins. They are C2, mercapto peroxide reductase -2, B6 No. L-3-hydroxyacyl coenzyme A hydrolase, B7, heat shock protein 27, No. B8 pyruvate kinase isoenzyme M2, B9, A12 α enolase 3 - A13 ketoacid coenzyme A transferase enzyme B15 number of heat shock protein 60, ATP synthase, No. B16 and B17 guanylate binding protein β2-like. Bioinformatics analysis queries show differences in protein and antioxidant effects, induce apoptosis signal transduction pathways, molecular chaperone and energy metabolic pathways related. Conclusion: Irinotecan Kang the role of human colon cancer LoVo cell lines the peroxide reductase -2 mercapto other differences in protein expression; involved in anti-oxidation mechanism of action; prompted irinotecan Sport anticancer mechanism may be related to the role of the above mechanism related.
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