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Preparation and Study of Biological Effects of Oxaliplatin Long Circulation Nanoliposomes

Author: YanShiNeng
Tutor: ChenYuXiang
School: Central South University
Course: Biochemistry and Molecular Biology
Keywords: Oxaliplatin Long cycle nanoliposomes body Zeta potential Encapsulation efficiency In vitro release rate
CLC: R96
Type: Master's thesis
Year: 2011
Downloads: 87
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Abstract

Objective: Oxaliplatin long-circulating nanoliposome four preparation methods are compared , its preparation process to optimize the design to reduce oxaliplatin toxicity , improve its bioavailability , that the class drug subsequent research , drug industrial production and clinical trial applications to provide a solid foundation . Methods : In this study, the vacuum evaporation method , thin - film dispersion method , ether injection method and ethanol injection method four oxaliplatin long cycle nanoliposome of preparation of a comparative study , the drug samples obtained transmission electron microscopy observation , and drug sample particle size and zeta potential analysis , the encapsulation efficiency of the drug sample recovery and in vitro release rate comparison , select the optimal preparation to facilitate the preparation of the drug sample cell MTT assay is its inhibition rate. Results: After the biological characteristics of the sample , the ethanol injection oxaliplatin long cycle nano liposome drug samples prepared better than the other three preparation methods , the best prescription oxaliplatin : natural soy phospholipids : cholesterol: polyethylene glycol 2000 proportion 1:2:1:1 concentration oxaliplatin 0.01g as a standard control , agitation ca about 40min . Prepared by this method to get the particle size of the the long cycle nanoliposome drug samples for 39.73nm Zeta potential - 45.3mV , drug encapsulation efficiency was 42.05% , the recovery was 100 % 72h in vitro release rate of 36.6% for the optimal. Cells by MTT assay results showed that the inhibitory rate of the drug concentration of the sample group over 1001μg/ml , 72h are up more than 90% of the 150 μg group reached 97.2% . Conclusion : The four preparation methods were successfully prepared Oxaliplatin long-circulating nanoliposome comprehensive comparison study on the biological characteristics of the sample , drug samples obtained by the ethanol injection method is better than the other three preparation methods . The method of preparation of the drug sample steady release within 72h shows better controllability than the positive group , long-lasting and stable persistence .

Preparation and Study of Biological Effects of Oxaliplatin Long Circulation Nanoliposomes

Abstract

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