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Culture of Sprague-Dawley Rats’ Neural Progenitor Cells in Vitro and Effects of EGF, bFGF, LIF

Author: PanLu
Tutor: ShengWeiBin
School: Xinjiang Medical University
Course: Surgery
Keywords: SD rat neural progenitor cells Epidermal growth factor Fibroblast growth factor Leukemia inhibitory factor Proliferation
CLC: R651.2
Type: Master's thesis
Year: 2010
Downloads: 40
Quote: 0
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Abstract


Objective: cultured and expanded in vitro hippocampus of neonatal Sprague-Dawley (SD) rats , to obtain a certain number of neural progenitor cells ( Neural the progenitor of cells , NPCs) NPCs growth was observed , analysis of the epidermal growth factor ( EGF ) , fibroblast growth factor (bFGF), leukemia inhibitory factor (LIF) independently or in combination to the proliferation of NPC . Method : mechanical separation neonatal SD rat hippocampus were cultured for 3 generations EGF / bFGF sensitive of NPCs , etc. is divided into six experimental groups were added EGF, bFGF, EGF LIF, bFGF LIF, bFGF EGF, bFGF EGF LIF to continue to foster (60 d) at the end of the ninth generation cells were identified and counted , to compare the number of cells . Results : the separation of (1) the mechanical method to get the NPCs in vitro suspension nervous ball of long-term culture amplification ; (2) EGF , EGF LIF group of NPCs train to the end of the 6th generation can not continue passaged culture ; remaining four groups continue to pass cultured still visible to the end of the ninth generation Nestin antibody - positive neurospheres formed, and partakers of the potential into the the three nerve terminal cells ; ( 3) cell culture is the end of the ninth generation , EGF bFGF group compared to the number of cells bFGF group , but bFGF, bFGF and between LIF group the EGF of bFGF , cell number between the EGF of bFGF the LIF group differences were not statistically significant ( P gt; 0.05 ) . Conclusion : SD rats NPCs can be long-term proliferation in vitro passage , its growth in the form of suspended neurospheres and differentiation potential ; the SD rats NPC cultured in vitro proliferation combination of EGF and bFGF stimulation than alone EGF or bFGF, alone is greater than the separate application of bFGF EGF ; LIF the SD rats NPC vitro proliferation of no effect .

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CLC: > Medicine, health > Surgery > Of surgery > Head and Neurosurgery > Spinal cord
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