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Differentiation Factor Expression and Regenerated β Cells in the Remodeling Period of Rat Pancreatic Islet

Author: XuRong
Tutor: DeWei
School: Nanjing Medical University
Course: Biochemistry and Molecular Biology
Keywords: Islet remodeling ngn3 pax4 nkx6.1 STZ
CLC: R587.1
Type: Master's thesis
Year: 2010
Downloads: 38
Quote: 0
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Abstract


Objective: In order to investigate the effects remodeling period ? Sources and mechanisms of cell differentiation . Modeling : STZ , the rats were divided into normal group ( control ) , STZ treated group (STZ), with respect to their blood sugar levels , body weight and pancreas weight observed statistics . We observed some key differentiation transcription factor pdx1 of ngn3 pax4 Nkx6.1 in the control group and the treatment group cellular localization by immunofluorescence double labeled observed after STZ treatment differentially expressed by western blot . Results observed after STZ treatment, blood glucose transient increased islet structural damage , extreme reduction in the number of islet β-cells , and then gradually recover . Islet ? Cells after STZ treatment , The number increased, at the same time begin to express a marked differentiation transcription factor ngn3 quantitative results show ngn3 protein expression was significantly increased . Treated rat islets ? Cells and beta cells significantly high expression of transcription differentiation factor pax4 the same time the the protein quantitative results showed that the expression level of absolute elevated . Nkx6.1 is located in the pancreatic β cells . Remodeling in rats after conclusion STZ treatment ngn3 high expression in islet ? Prompted islet cells ? Cells may be a source of islet beta cells . Normal rat group reshape Islet ? Cells also express pax4 , the treatment group pax4 expression was significantly elevated , and this can trigger ngn3 high expression and start islet ? Cells to generate pancreatic β cell process , may reshape the rat STZ treatment of hyperglycemia self - recovery reasons. Therefore study the remodeling phase the islet ? Cells can be used as a Get islet β-cells in the treatment of diabetes research directions .

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CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetes
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