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Research purposes by observing the changes of the HO metabolite concentrations in the plasma of patients with diabetic nephropathy different periods as well as with nitric oxide, nitric oxide synthase, superoxide dismutase, malondialdehyde oxidative stress indicators, and to explore the HO / CO system indicators of clinical significance in diabetic nephropathy, diabetic nephropathy, development provides a theoretical basis. Research methods. Specimens collected: the case inclusion criteria collected from 127 patients with type 2 diabetes, according to The urine microalbumin the level of type 2 DM patients were divided into three groups: diabetic group (DM), 45 patients were male 26 cases, female 19 cases; early diabetic nephropathy (DM Ⅰ group), 42 cases, including 24 males and 18 females; clinical diabetic nephropathy (DM II), 40 cases, including 17 males and 23 females. 30 cases of physical examination health healthy subjects. Collect blood samples and record their age, gender, disease duration, blood pressure and general clinical data. Indicators will be measured: fasting blood glucose (FBG), glycated hemoglobin, triglycerides (TG), total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL), bilirubin, uric acid ( UA), blood urea nitrogen (BUN), creatinine (Cr) Determination of Biochemical. Plasma superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide (NO), nitric oxide synthase (NOS), carbon monoxide (CO), serum iron determination. The urine microalbumin Determination. 3. Statistical analysis: SPSS11.5 statistical software for processing multiple comparison of the number of samples, using one-way ANOVA and non-parametric tests. Analysis of the correlation between the two variables, pearson linear correlation analysis or spearman's rank correlation analysis. The inspection level a = 0.05. Results HO / CO system: each group bilirubin concentration showed a downward trend, DM Ⅰ group than in the control group (P lt; 0.05 or P lt; 0.01); DM II group than in the DM Ⅰ group (P lt; 0.05 or P lt ; 0.01). Serum iron, plasma CO showed a downward trend, DM Ⅰ group than in the control group (P lt; 0.05), DM II group than in the DM Ⅰ group (P lt; 0.05). The plasma CO with BUN, Cr, urine Alb, MDA showed a negative correlation (P lt; 0.01 or P lt; 0.05); the total bilirubin course of BUN, Cr, urine Abl was negatively correlated (P lt; 0.01 or P lt; 0.05); direct bilirubin and course of the disease, Cr, urinary Alb was negatively correlated (P lt; 0.01 or P lt; 0.05); indirect bilirubin and course of the disease, BUN, Cr, urine Alb was negatively correlated (P lt; 0.01 or P lt; 0.05); serum ferritin and urinary Alb was negatively correlated (P lt; 0.01). Changes in the diabetic group SOD, MDA indicators: blood SOD levels downward trend, the DM Ⅰ group than in the control group (P lt; 0.05); DM II group than in the DM Ⅰ group (P lt; 0.05). Plasma MDA concentration tended to increase, DM Ⅰ group was significantly higher than that in the control group (P lt; 0.01), DM Ⅱ group was significantly higher than the DM Ⅰ group (P lt; 0.01). SOD / MDA ratio declining the DM I group was significantly lower than the control group group (P lt; 0.01), DM Ⅱ group was significantly lower than DM Ⅰ group (P lt; 0.01). SOD / MDA Ratio with the course of the disease, FBG, TG, TC, BUN, Cr, urine Alb was negatively correlated (P lt; 0.01 or lt; 0.05); plasma CO was positively related (P lt; 0.01). 3. NO / NOS system: plasma NO, NOS concentration the: DM Ⅰ group than DM group (P lt; 0.05); the DM II group was significantly lower than the DM Ⅰ group (P lt; 0.01). The concentration of NO in blood and urine Alb, MDA was negatively correlated (P lt; 0.05 or P lt; 0.01), and NOS showed a positive correlation (P lt; 0.01); blood the NOS and urine Alb was negatively correlated (P lt; 0.05). Conclusions in patients with diabetic nephropathy plasma CO, bilirubin, serum iron concentration showed a downward trend, blood NO, NOS increased in early diabetic nephropathy, and clinical diabetic nephropathy reduce CO / of HO system and NO / NOS system may by oxidation The stress involved in the development of diabetic nephropathy.
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