|
Objective: To take advantage of changes in gene chip technology detects ulcerative colitis rat gene expression profiles, screening analysis differences expressed genes, and to investigate the mechanism of pathogenesis of ulcerative colitis and Uygur medicine the SIPA in accordance kuijiean. Methods: Wistar rats were randomly divided into normal group, the model, using 2,4 - dinitrochlorobenzene (2,4-dinitrochlorobenzene, DNCB) plus acetic acid complex method to copy a rat model of ulcerative colitis; then ulcerative colitis rats were randomly divided into model group, saline negative control group, of Uygur medicine SIPA by kuijiean large, medium, small doses of the intervention group and 20 days were given the drug intervention. Application Crystal Core? 27K Rat Genome Array chip detection of changes in gene expression of rats screened differentially expressed genes and biological information analysis. Using relative quantitative fluorescence real-time PCR gene mRNA level validation expression on the part of differences, to determine the results of microarray reliability. Results: The model rats of symptoms, signs, colon tissue specimens, biopsy results are in line with the standard model of ulcerative colitis; gene chip screening results show that: the model group compared with the normal group, screened differentially expressed genes 1054 667 upregulated and 414 gene expression are down compared to the negative control group in each dose group with saline; 387 downregulated, 168 in each dose group and the saline negative control group compared expression are raised. Gene functional pathway enrichment analysis showed: the differentially expressed genes include genes related to cell signaling, metabolism, immunity, inflammation and other life activities, dig out some genes related to the incidence of ulcerative colitis for further information, for example, : TLR2, TLR6, NF-κB, IL-1β, CCL3 and TGF-β1 in. The applications relative quantitative fluorescent real-time PCR technology for partial differential gene mRNA level of verification, the results show that the real-time PCR results and the microarray results have good consistency, further confirmed the reliability of the results of the chip. Conclusion: DNCB and acetic acid complex method successfully copied the rat model of ulcerative colitis. The Uygur medicine SIPA By kuijiean has a good effect on rats with ulcerative colitis, can lead to significant changes in ulcerative colitis rat gene expression profiles. UC occurrence dimensional drug SIPA By kuijiean ulcerative colitis may change with the expression of many genes. Differentially expressed genes for further study will help to clarify the mechanism of ulcerative colitis and Uighur Medicine provide new targets and strategies the Cipa By mechanism kuijiean, for the treatment of ulcerative colitis.
|