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Objective: To research β2-AR gene 5'-regulatory region SNP the Seoul family with essential hypertension (EH) and with the the Xinjiang Kazakh and dimensional; explore the genetic susceptibility of different races EH by comparison with the Uyghur population mechanism. Legislation: (1) using the polymerase chain reaction (PCR) and single-base extension with MALDI-TOF-MS (Matrix assisted laserdesorption ionization time of flight mass spectrometry) analysis combined with technology to detect the Xinjiang Kazakh population 150 primary hypertensive patients and 150 normal controls and Xinjiang Uygur population of 100 patients with essential hypertension and 100 normal controls β2-AR gene 5'-regulatory region of six SNP loci, using biochemical analyzer lipids and other biochemical markers. (2) using SPSS16.0 statistical software for statistical analysis, calculate the genotype frequencies of the two groups to confirm compliance with the Hardy-Weinberg equilibrium; χ2 test was used to compare the allele and gene frequencies between the two groups; clinical indicators t-test was used to compare ; hypertension risk factors using logistic regression; linkage disequilibrium and haplotype analysis SHEsis software. Results: (1) in Xinjiang Kazak and Uyghur population six sites of the β2-AR gene 5'-regulatory region (-893T / G) rs2053044 (-1023G / A), rs17108803, rs12654778 (-654G / A) , rs11168070 (-468C / G), rs11959427 (-367C / T), rs2895795 (-1429T / A) gene polymorphism. rs2053044 genotype GG, GA, AA and allele G, A frequency distribution in the EH group and normal control group between the two ethnic difference (P gt; 0.05); rs12654778, rs2895795, rs17108803, rs11959427 bit point The difference between the genotype and allele frequency distribution of two ethnic Kazakh and Uighur EH group and the normal control group without statistical significance (P gt; 0.05). rs11168070 genotype CC, CG, GG and allele C, G frequency distribution in the Kazakh EH group and normal control group significant difference (P lt; 0.05); (2) Hasake do linkage disequilibrium and haplotype analysis found strong linkage disequilibrium exists in Kazakh EH group, the control group population, the EH group rs11168070, rs2053044, rs2895795 locus gene polymorphism in strong linkage disequilibrium coefficient D 'were 0.9122,1.000,0.7689 correlation coefficient r2 were 0.1273,0.1606,0.3900 normal control group rs11168070, rs12654778, rs17108803 locus gene polymorphisms in linkage disequilibrium. The haplotype GTCCAT, GACTGT ATGCGT ATCTGT ATGTGT GTCCGT GTCTAT, GACCAT, GTCTGT EH which GTCCAT GACTGT ATGCGT is harmful factors, ATCTGT, ATGTGT, GTCCGT, GTCTAT, GACCAT, GTCTGT protective factors . (3) Kazakh EH group systolic blood pressure, diastolic blood pressure, mean arterial blood pressure, body mass index, total cholesterol was significantly higher than the Uighur EH group, overweight, obesity, abdominal obesity, hyperglycemia, abnormal lipid metabolism the two nationalities EH common risk factors, in addition to too little intake of vegetables also Kazakh EH risk factors, and has nothing to do with the Uighurs. Industry: (1) the Xinjiang Kazak and Uyghur population p2-AR gene 5'-regulatory region of five loci rs2053044, rs17108803, rs12654778, rs11959427, rs2895795 gene polymorphism exists, the genotype and allele frequency distribution in two nationalities in the EH group and normal control group was statistically significant; (2) rs11168070 locus genotype and allele frequency distribution Kazakh EH group and normal control group was statistically significant; (3) Hasake, rs2053044, rs17108803, rs12654778, rs11168070, rs11959427, rs2895795 polymorphism loci there is a strong linkage disequilibrium, the six who constitute the haplotype GTCCAT, GACTGT, ATGCGT Xinjiang Kazakh EH risk factors, the while ATCTGT ATGTGT, GTCCGT, GTCTAT, GACCAT, GTCTGT protective factors.
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