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Effects of Orexin-A on Rat Blood Pressure and Plasma Adrenocorticotropic Hormone and Corticosterone Levels

Author: ChenYiBing
Tutor: KongXuLi；ZhangChao
School: Zhengzhou University
Course: Physiology
Keywords: orexin ACTH Blood pressure Corticosterone ELISA
CLC: R33
Type: Master's thesis
Year: 2008
Downloads: 33
Quote: 0
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Abstract


Preface: stress-induced physiological changes including autonomic and neuroendocrine activities change following hypothalamic - pituitary - adrenal cortex (HPA) axis activation and the resulting glucocorticoid (GC) increased secretion of important characteristics. Hypothalamus as an important neuroendocrine and autonomic regulation center, through the integration of in vitro and in vivo stress stimulation of endocrine and autonomic nervous activity changes in the maintenance of homeostasis and adaptive response of the body plays an important role. The Orexin lateral hypothalamus region of a Class (lateralhypotahalmic area LHA), and the dome peripheral zone of the newly discovered neuropeptide orexin-A and orexin-B two highly homologous peptides. Orexin receptor orexin-1 receptor (orexin type 1 receptor, OX1R), and orexin-2 receptor (orexin type 2 receptor, OX2R), they belong to the G protein-coupled receptors (G protein-coupled receptor, GPCR) family. orexin neurons in the lateral hypothalamic area and vault peripheral zone, the fiber is widely projected to the entire central nervous system involved in the regulation and integration of the different physiological functions, currently known to participate in the regulation of physiological activities including feeding, digestion, energy metabolism balance , sleep / wake cycle, drug dependence. Orexin neurons in the hypothalamic paraventricular nuclear (paraventricularhypothalamic nucleus, PVN) and other autonomic nervous and endocrine activity regulation center projection more and more attention has been paid. And the number of experimental evidence that the Orexin possible as a neuromodulator involved in the body's stress response. We assume that Orexin-A may be involved in stress-induced cardiovascular response in rats and pituitary - adrenal axis activity changing the destination: the observed lateral ventricle injection of Orexin-A conscious rats tail artery systolic pressure (tail arterialsystolic blood pressure, TBP) and pituitary - adrenal axis. Methods: 10 healthy adult male Sprague-Dawley rats were randomly divided into two groups, the establishment of the lateral ventricle sterile surgical intubation, recovery after 10 days, a group of injection of 10μg orexin-A (dissolved in of 5μ1 saline), and the other group injection 5μ1 saline as a control. Using non-invasive tail arterial blood pressure measurement system before the measurement intracerebroventricular injection of 10 minutes and 30 minutes after the injection of TBP changes After measurement, the rats were sacrificed quickly cryogenic centrifuged and the plasma stored at -80 ℃, using enzyme-linked immunosorbent assay (enzyme-linked immunosorbantassay ELISA) analysis of rat plasma adrenocorticotropic hormone (adrenocorticotropichormone, ACTH) and corticosterone concentration. Results: 1. Intracerebroventricular injection of 10μg orexin-A may be significantly increased in rats TBP 10 minutes before the injection of TBP 117 ± 2.5mmHg, 10 minutes after the injection, 20 minutes and 30 minutes TBP were raised to 136 ± 6.1mmHg, 1344 ± 5.3mmHg, 130 ± 3.4mmng (P <0.01). Compared with the lateral ventricle injection of saline, lateral ventricle injection Orexin-A significant increase in plasma ACTH in rats (P <0.01) and corticosterone (P <0.01) concentrations. Conclusion: lateral ventricle injection Orexin-A can increase the rat TBP increases in plasma ACTH and corticosterone levels.

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