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- Members of a1- adrenergic receptor (a1-adrenoceptor, a1-AR) is a G protein-coupled receptor family , widely distributed in the body's various organs , tissues and cells . And mediate a variety of physiological effects , such as urinary tract smooth muscle contraction , vasoconstriction , glycogenolysis , the cardiac inotropic change , along with the progress of recent years , pharmacological and molecular biology techniques , a1- adrenergic receptor subtype classification and distribution has gradually clear . This is a great help in clinical medicine . Objective: To investigate a1 adrenergic receptor subtypes in the rat ureter , distribution characteristics , under paragraphs (a1-AR) . Methods : This article radioligand binding assay using GraphPad PRISM 5.01 software to calculate the density of receptors and their subtypes , to study the a1 adrenergic receptor (a1-AR) subtypes in the rat ureter , characteristics of paragraphs distribution . All data are x ± s and statistically analyzed by t-test , P lt ; 0.05 difference significant sexual standards . Statistical analysis using SPSS11.5 software . Conclusion: analysis by saturated test results a1-AR in rat ureter each segment binding site is a non - uniform distribution , the lower ureter Bmax 14911 ± 1253 fmol / mg , and was significantly higher than that of the previous paragraph ( 9077 ± 211fmol/mg ) and middle (9164 ± 158 fmol / mg), the distribution of the upper and the middle of the difference was not significant . Crude membrane specimen after CEC pre-incubated , A1 -AR and [ 125I] - HEAT of the maximum binding capacity Bmax than the control group decreased approximately 64%. Competitive inhibition test results show that in a1-AR subtype - specific antagonist ( 5- MU , Cyclazosin BMY7378 ) exist a1-AR [125I ] - HEAT the maximum binding capacity Bmax varying degrees of decline . Thus we have come to the conclusion a1-AR is higher than the density of the lower ureter and middle , but the Kd values ??and Hill coefficients and there is no difference . Inhibit the reaction shows that there are three kinds of a1-AR subtypes , namely of a1A, a1B and a1D-AR subtypes in the rat ureter through a single point of competition , but the a1D-AR subtype a1A-AR subtype distribution significantly more than a1B-AR subtypes , slightly higher than the a1A-AR subtypes a1D-AR subtypes .
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