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Study on the Animal Models of Colon Motion’s Kinetic Injury Caused by Ischemia-reperfusion

Author: WangHong
Tutor: TangFang
School: Tianjin Medical University
Course: Traditional Chinese Medicine
Keywords: intestinal ischemia-reperfusion limb ischemia-reperfusion colon motion colon mucosa Ca2+-ATPase activity
CLC: R-332
Type: Master's thesis
Year: 2009
Downloads: 27
Quote: 0
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Abstract

Objective:The gastrointestinal motility function is one of the most significant physiological functions of the digestive system. The clinical symptoms of abdominal distention, nausea, vomiting, diarrhea and constipation as well as depression and debilitation which belong to the category of the spleen and stomach disease of Traditional Chinese medicine. According to the research,IR can lead to depression, less actions, matted fur, abdominal debilitation and also the disorder of colon motion of model animals, which prompt the model of IR can analogue the symptoms of Traditional Chinese medicine:choking of the middle energizer, stasis of the movement of qi, the sag of spleen qi, super inverse of stomach qi.Setting up 2 kinds of animal models: limb ischemia-reperfusion and intestinal ischemia-reperfusion explore the regularity of different reperfusion time injury in rats and find out a better model for functional gastrointestinal disorders and disorders of gastrointestinal motility by observing the mental status and vital signs;recording the average contractile amplitude of colonic muscle strips by a multiscriptor; detection of the Ca2+-ATPase and observing the morphological changes of intestinal mucosa,colon mucosa and capital organs through microscopy.Method:1.Setting up the model of intestinal ischemia-reperfusion:The superior mesenteric arteries in the three groups were occluded for 45 minutes and reperfusion for 6 hours 24 hours and 48 hours. And then different groups were:reperfusion for 48 hours after occluding the superior mesenteric arteries for 60 minutes and 75 minutes.2、Setting up the model of limb ischemia-reperfusion:using rubber band hypodesis the limbs of rats for 3 loops with same tensile force 300g. for 3 hours, and then reperfusion for 12hours,18hours and 24hours3、Observing the morphological changes of intestinal mucosa,colon mucosa and capita organs through microscopy.4、Recording the average contractile amplitude of colonic muscle strips by a multiscriptor. 5、Detection of the Ca2+-ATPase of cells of colonic smooth muscle. Results:1、The model of intestinal ischemia-reperfusion:After extending the time of reperfusion,the mental status and vital signs became worse and there were changes in the small intestine mucosa which was in the ischemia-45min group.But the contractile amplitude of colonic muscle strips ascensused in the C45-48 group.After extending the time of reperfusion from 45 minutes to 60 minutes,both small intestine and colon mucosa had pathological changes, and the mental status and vital signs became worse and worse; average contractile amplitude of colonic muscle strips decreased in the C60-48 group, there were differences between this group and control group;while the colonic muscle strips of group C75-48 became thin,so the amplitude could not be record.There was no differences of activity of Ca2+-ATPase between the group of ischemia 75min and the control group.2、The model of limb ischemia-reperfusion:Extending the time of reperfusion, there were pathological changes on the small intestine mucosa,colon mucosa, heart, lung, and kidney. In comparison with the control group, the average contractile amplitude of colonic muscle strips was decreased in the group of reperfusion 12 hours and the group of reperfusion 18 hours (P<0.05, P<0.01);while the colonic muscle strips of group of reperfusion 24 hours became thin and could not record the amplitude. The activity of Ca2+-ATPase was ascensus in the group of reperfusion 12 hours, while it decreased in the groups of both reperfusion 18 hours and reperfusion 24 hours.Conclusion:The two kinds of models had their own dynamic regulars because of different making methods, but they can both injury the colon motion, so they can be used as models to analogue the functional gastrointestinal disorders and disorders of gastrointestinal motility.The model of limb ischemia-reperfusion is better.

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