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Objective: To investigate Qiweibaizhu Powder on rat small intestinal HRV infection and iEL IκB.NF-κB impact resistant HRV further clarify the mechanism of the party. Method: 1. Using HRV suspension by the oral route infection in 5-day-old suckling mice, rat feces taken by the monoclonal antibody enzyme-linked immunosorbent assay (McELISA) confirmed rotavirus antigen, the establishment of suckling mice infected with HRV diarrhea model. Two would suckling mice were randomly divided into model group at Waterloo (MG), Qiweibaizhu Powder Group (BG), ribavirin group (ZG), inhibitor group (YG), Atractylodes Powder Plus inhibitor group (BYG), and the establishment of the normal control group (NG), a total of six groups. Suckling mice in each treatment group were dropped in the appropriate medication by mouth 50μl / (only · times), 3 times / day. Normal control group and model group oral instillation equivalent cell maintenance medium. 3 (a) treating the 5th suckling mice were sacrificed after HE staining of drugs on rat small intestinal mucosa protective effect; ELISA were determined before and after drug intervention HRV stool suckling mice and suckling mice infected serum levels of IL-8 . (2) after treatment of neonatal rats were 15min, 1h, 2h, 6h, 12h, 24h, 32,48 h taking rat small intestinal mucosa was determined by immunohistochemistry drugs on rat small intestinal tissue IκBαNF-κB expression; with immunomagnetic separation neonatal rat small intestinal iEL CD3 T cells was measured by RT-PCR method iEL CD3 T IκBα,-κB mRNA; Western Blotting determined iEL CD3 T cytoplasm IKBα and iEL CD3 T nucleus of NF-κB protein content. Results: 1. Suckling mice infected with HRV 5 days of continuous treatment, BG rat droppings ZG HRV clearance rate and the same, with no statistically significant difference compared ZG (P gt; 0.05), but significantly better than YG and BYG, the difference was statistically significance (P lt; 0.05). 2.HE dyeing, BG suckling mice significantly improved intestinal mucosal lesions, respectively, and ZG, YG, BYG, the difference was statistically significant (P lt; 0.05). 3 5 days after treatment in each group rat serum interleukin comparison Qiweibaizhu powder group were significantly lower than IL-8 modules, the difference was statistically significant (P lt; 0.05). 4 Qiweibaizhu Powder treated rat small intestinal tissue cytoplasm IκBα protein expression and iEL in CD3 T cells IκBαmRNA expression increased with ribavirin group, inhibitor group, Qiweibaizhu Powder plus inhibitor group (P lt; 0.05 ), and its expression in 12h after waning. 5 Qiweibaizhu Powder can regulate small intestinal mucosa of suckling mice infected nuclei NF-κB protein expression and iELCD3 T cells, NF-κB mRNA expression, and its content is lower than the model group (P lt; 0.05). 1h, 24h, 32h, 48h below ribavirin group P lt; 0.05; higher inhibitor group, Qiweibaizhu Powder plus inhibitor group (P lt; 0.05). Conclusions: 1. Qiweibaizhu Powder HRV infection can reduce neonatal rat serum IL-8 levels, removal of infected suckling mouse intestinal HRV, reduce neonatal rat small intestinal lesions. 2 Qiweibaizhu Powder HRV infection can promote neonatal rat small intestinal tissue and iELCD3 T cytoplasm IκBαmRNA and IκBα protein expression, and regulation iELCD3 T nucleus of NF-κB, involved in signaling pathways downstream molecular events induced.
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