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Preparation and Characterization of Monoclonal Antibody Against TACI

Author: LiuYing
Tutor: JinBoQuan；YangZuo
School: Fourth Military Medical University
Course: Immunology
Keywords: TACI mAb Flow cytometry Distributed
CLC: R392
Type: Master's thesis
Year: 2003
Downloads: 62
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Abstract

Tumor necrosis factor superfamily (the superfamily of tumor necrosis factor, a member of TNFSF) and tumor necrosis factor receptor superfamily (TNFRSF) is increasing, they are not only widely variety of physiological functions in mammals, but also closely related to the pathogenesis of many diseases. Transmembrane activator and calcium tune pro cyclophilin ligand interacting molecules (transmembrane activator and calcium modulator and cyclophilin ligand (CAML) interactor, TACI) is the recently discovered TNFRSF in new members irritants and B cells (B both lymphocyte stimulator, BlyS, also known as BAFF) and a proliferation-inducing ligand (a proliferation inducing ligand, APRIL) ligand binding, mediated humoral and cellular immunity. B cell maturation molecules (B cell maturation molecule, BCMA) with TACI competition to combine BlyS and APRIL. Recently found BAFFR specific receptor for BAFF. TACI is a type I transmembrane protein expressed on the cell surface of mature B cells and activated T. Murine type II collagen-induced arthritis model, the application of TACI-Fc fusion protein the to blocking BlyS and APRIL with the cell membrane surface TACI or BCMA combination, thereby inhibiting the humoral and cellular immune function, significantly reduced arthritis pathological changes . BlyS transgenic mouse performance for B-cell aggregation, activation, secretion of anti-DNA antibodies and other autoantibodies (such as rheumatoid factor, RF), and the emergence of autoimmune lupus nephritis and other symptoms. TACI-Fc in vitro specific inhibition BlyS mediated Journal of the Fourth Military Medical University, Chinese summary B-cell proliferation and activation of T cells; reduce the number of activated B cells in vivo can inhibit the formation of germinal centers, to prevent the development of nephritis to ease the symptoms of SLE, and increased survival. The above results show that the soluble TACI may play a therapeutic effect by interfering with secretion of autoantibodies, showing good prospects for the development of. Preparation of anti-TACI monoclonal antibodies provide an important means will further study the the TACI molecular structure and function, and some autoimmune diseases. E3.5 and E3.7 is people TACI dry. The fusion protein was used to immunize BALB eight mice get to two mAbS. The study found, the two mAbS identify molecules selectively expressed on allogeneic antigen, PHA, PMA activated lymphocyte surface, including CD4 Wo CDS + T cell subsets. E3.5 identified by immunohistochemistry, found recognition molecule expression 38Wb fetal dermis layer. In tonsil germinal center also expression.

Preparation and Characterization of Monoclonal Antibody Against TACI

Abstract

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