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Magnolia warm soup originated within trauma apologetics' by Magnolia, dried tangerine peel, licorice, Poria, grass cardamom, woody, dried ginger Qiwei herbs, the research method of quality control of the party, preparations extraction process, preliminary Pharmacodynamic and pharmacokinetic study. Establish of the the Alpinia herbs Zhongshan Jiang Su and the cardamonin content HPLC method linear range -1 and 3.0 ~~ 60.0μg of 6.0 ~ 120.0μg · mL · mL of -1 (r ≥ 0.9991), the average recovery was 99.7% and 99.8%, respectively; Magnolia temperature in the soup Six indicators ingredients while measuring method , the measured Alpinetin, honokiol, cardamonin the costunolide, dehydroepiandrosterone lactone and Magnolol of the linear range were 3.0 ~ 60.0μg · mL -1 , 7.4 ~ 148.0μg · mL -1 , 0.75 ~ 15.0μg · mL -1 , 5.50 ~ 110 .0 μg · mL -1 , of 7.80 ~ 156.0μg · mL -1 and 18.50 370.0μg · mL -1 (r ≥ 0.9991), the average recoveries were 99.1%, 96.6%, 100.7%, 96.1%, 99.2% and 99.8%; established a the Magnolia temperature in glycyrrhizic acid content determination method in the soup, the linear range of 25.0 ~ 500.0μg · mL -1 (r = 0.9997), the average recovery was 97.0%; established Magnolia warm soup in the Determination of hesperidin linear range for of 14.0 ~ 280.0μg · mL the -1 (r = 0.9995), the average recovery was 97. 5%. This study to establish more comprehensive quantitative analysis method to control the quality of the party. The Magnolia temperature in the soup extract yield and index components Mountain Jiang Su, cardamonin and the costunolide dehydrogenase lactone and honokiol, magnolol, glycyrrhizin, hesperidin The content of the indexes, determine the best extraction solvent 95% ethanol; orthogonal experimental design to optimize the extraction process in the amount of solvent, extraction time and extraction times three factors. By comprehensive evaluation method to determine the optimum process: a full 10 times the amount of 95% ethanol reflux extraction twice, each time 1.5 h, the extraction process can be optimized to provide a basis for further improvement of the dosage form. Ulcer index and ulcer inhibition rate pharmacological evaluation, and research the Magnolia temperature in the soup protective and therapeutic effect on rats, mice of different models of gastric ulcer. The experimental results show that, compared with the model group, different doses of the test of gastric ulcer have better protective and therapeutic effect. Magnolol in rat plasma by HPLC method, as an internal standard, plasma samples were extracted with ethyl acetate to ibuprofen treatment last-sample analysis. Column Scienhome C 18 (250 mm × 4.6 mm, 5 μm); mobile phase methanol 0.5% acetic acid aqueous solution (75:25, v / v), the column temperature was 30 ° C. The flow rate was 1.0 mL · min -1 , the detection wavelength of 225 nm, the injection volume of 20 μL. The linear range of 0.08 ~ 8.0μg · mL -1 (r gt; 0.991), average extraction recovery was more than 70.0% of the intra-day RSD were less than 14.0%. The established method of biological samples and analysis requirements. Rats by oral administration the Magnolia temperature in the soup suspension Honokiol pharmacokinetic study. Honokiol T max , approximately 1.09 h, C max approximately 0.52μg · mL of -1 , the AUC -t for 2.9 μg · h · mL -1 the t 1/2α for 0.84 h, t 1/2β 13.9 h, the body process in line with the two-compartment model.
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