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This thesis, a study of traditional Chinese medicine active component analysis methods, especially for the analysis of trace target is divided into three chapters, its main contents are as follows: Chapter 1: Summary, Salvia and rhubarb, is a traditional Chinese medicine in China, Its effectiveness is very wide. This section, the efficacy of these two drugs, extraction and analysis methods of a simple overview of. In addition, the current method of extraction and preconcentration status quo overview and homogeneous liquid-liquid extraction method Offline enrichment tanshinones substances, rhubarb anthraquinone class line preconcentration by micellar electrokinetic capillary chromatography - micelle collapse The substance of the new method. Chapter 2: the application of homogeneous liquid-liquid extraction the three tanshinone material of Chinese medicine Salvia enrichment detected by high performance liquid chromatography. Experiments using methanol as solvent extraction Salvia in three Tanshinone substance as a sample solution, with chloroform as the extraction reagent, sodium chloride as the phase separation reagent. In methanol - chloroform uniform solution with no stars interface, ie at the beginning of both the contact area between the infinite, i.e. no mechanical shock can realize the transfer of the target substance, sodium chloride was added after centrifugation The test tube will appear at the bottom of a small droplet. TCM active ingredient extracted from a large volume of the alcohol solvent (ml) to the micro-volume of chloroform phase (μl) in order to achieve effective enrichment. The present part of the study the impact of the various experimental conditions for droplet volume and the enrichment factor and the extraction rate, lml extract, chloroform was added 60μl of 5% (w / v) aqueous solution of sodium chloride salt 3ml, salt content, The enrichment factor can reach 60-100 fold, the extraction rate in the 92% -97%, the reproducibility RSD LT of the droplet volume; 10% (n = 5). The enrichment method is simple and efficient, and do not need to add special equipment can quickly realize the microanalysis, suitable for the pre-treatment analysis of Chinese herbal medicines. Chapter 3 in micellar electrokinetic capillary chromatography, based on the establishment of a new enrichment technology - micelle collapse. The experiments on the composition of the background electrolyte types and salt content of salt in the sample, the concentration of sodium dodecyl sulfate (SDS), and injection time, the conditions were optimized, rhubarb four anthraquinone substances were determined . The background electrolyte solution is 20mm SDS, 5mM borax, and 20% acetonitrile, the rhubarb extract solution was added to the diluted 10-fold after separation in the above-described background solution 88mmM sodium acetate and 5mM SDS. Emodin, rhein, chrysophanol and emodin enrichment factor 7,15,1 l and 82 times the peak height RSD% (n = 3) were 2.10,2.95,3.69 and 3.16, showing that weight good reproducibility.
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