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In recent years, the side effects of chemical drugs continue to emerge, looking for natural medicines are growing, traditional Chinese medicine for its unique efficacy, less toxicity to the attention of the international medical community. Traditional Chinese medicinal Schisandra has high medicinal value in traditional medicine, but by some of the impact of the natural environment, and some quality less than the required standards and limits the speed to enter the international market. Methods for testing the quality of Schisandra this Schisandra planting base specification, cultivate high-yield, high-quality, pollution-free new varieties, while establishing an efficient, accurate determination of the main ingredients in schisandra the track will be very necessary for Traditional Chinese Medicine in China material provide the basis for quality evaluation. This study to establish a high-performance liquid chromatography analysis method for simultaneous determination of four lignans from Schisandra compounds the effects of mobile phase composition and ratio, detection wavelength, column temperature, flow rate, and injection quantity measurement determine the best chromatographic conditions: mobile phase of acetonitrile - water gradient elution program :0-25 min acetonitrile: water = 50:50 (v / v); 25-35 min linear change to 70:30 (v / v), and keep for 5 min; 40-44 min and then a linear change to 80:20 (v / v); the DAD detector detection wavelength schizandrol, Deoxyschizandrin Schisandrin B 216 nm, and Schisantherin A 222 nm; column temperature of 25 ° C; flow rate of 0.8 mL / min; μL 10.0 μL of injection volume. Optimum chromatographic conditions, a good linear relationship of the four compounds standard curve, and the correlation coefficient of 0.9994-0.9998, and the detection limit of each component 0.04-0.05μg/mL average recovery was 90.23-95.24%, relative The standard deviation of 3.2-4.9%, and the determination on the the four lignans class compounds while. Determination of the method of analysis of a variety of organic acids compounds Schisandra experiment to establish a high-performance liquid chromatography, effects of mobile phase composition and the ratio of buffer concentration and pH values, detection wavelength, column temperature and flow rate measurement to determine the best chromatographic conditions: mobile phase of methanol -0.01 mol/LNaH2PO3 (pH = 2.8), gradient elution program :0-5 min methanol-0.01 mol / L NaH2P03 (pH = 2.8), the ratio of 2: 98 (v / v); ratio of 5-16 min both linear change to 40:60 (v / v); DAD detector, the detection wavelength was set at 0-4.7 min 210 nm ,4.7-5 .3 min 260 nm , 5.3 min, 210 nm; column temperature of 25 ° C; flow rate of 0.8 mL / min; injection volume μL 10.0 μL. Best chromatographic conditions, the the seven compounds standard curve linear relationship, the correlation coefficient of 0.9990-0.9999, the detection limit of each component 0.01-2.5μg/mL average recoveries for 90.29-102.5%, relative The standard deviation of 1.0-4.6%, and achieve rapid determination of a variety of organic acids, compounds. The results show that, sensitive and accurate the two simultaneous determination of the main ingredient in Schisandra analysis method.
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