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Objective: To observe Qili cardiac capsules heart failure cardiac function after myocardial infarction and apoptotic protein caspase-3 expression, explore Qili cardiac capsules mechanism of action for the treatment of chronic congestive heart failure. Methods: 60 healthy Sprague Dawley (SD) male rats, weighing 200-250g, ligating the left anterior descending branch of the left coronary artery in rats, rat model of heart failure after acute myocardial infarction (AMI), survival rats were randomly divided for the model group, the Qili cardiac capsules high, medium and low dose group (high, medium and low dose group) and captopril group, with another sham operation group. The Qili cardiac capsule after I powder 1.2 g / kg d, 0.6 g / kg D, 0.3 g / kg d treatment with captopril to captopril 25mg/kg d treatment, model group and sham operation group to the same volume of saline treatment after 4 weeks of the detection body weight (BW), hemodynamic changes, cardiac mass index (HMI), left ventricular mass index (LVMI), myocardial HE staining, immunohistochemistry and western- blot was used to measure apoptotic protein caspase-3 expression. Results: After 4 weeks of treatment, surgery rats showed varying degrees of tired lying, hair removal, weight loss, anorexia, loose stools and other symptoms that rats, left ventricular end-diastolic pressure (LVEDP) 16.65 ± 1.53 mmHg, model copy success. (2) Compared with model group, the the Qili cardiac capsules treatment group and captopril group can effectively reduce the weight of each treatment group (BW), heart mass index (HMI), left ventricular mass index (LVMI), Qi Lepidii cardiac capsules, the middle dose group and captopril treatment group difference was not statistically significant. 3 Compared with model group, the the Qili cardiac capsules treatment group and captopril group can effectively reduce the left ventricular end-diastolic pressure (LVEDP), the effective increase in left ventricular end-systolic pressure (LVSP), left ventricular pressure increase and decrease in speed (± dp / dt max), high-dose group. (4) The results of immunohistochemistry Tip: the surgery group caspase-3 protein expression in a significant increase compared with the normal group, model group, the most significant; compared with the model group, captopril, Qili cardiac capsules treatment group can effectively reduce the caspase-3 protein, Qili cardiac capsules treatment group into a dose-dependent manner. Western-blot results: Compared with normal group, model group, the Qili cardiac capsules treatment, low-dose group caspase-3 protein expression in a significant increase compared with the sham-operated group; compared with the model group, the the Qili cardiac capsules treatment of high-dose group and captopril group of caspase-3 protein expression were significantly lower. Conclusion: This study diminished myocardial contractility that ligation of the left coronary artery was successfully copied the rat model of heart failure, confirmed the Qili cardiac capsules treatment of AMI rats with heart failure can significantly improve the general and cardiac function, and found Qili heart capsule could down-regulate the apoptotic protein caspase-3 expression levels, suggesting that it can effectively inhibit heart failure after myocardial apoptosis. Speculate Qili cardiac capsules can effectively improve heart function mechanism of action may be related to inhibition of myocardial remodeling and myocardial cell apoptosis.
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