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Complete Genomic Sequence of Bovine Viral Diarrhea Virus (BVDV) ZM-95, a Pestivirus from Swine

Author: ZhangQingZuo
Tutor: TuChangChun
School: Quartermaster University of PLA
Course: Preventive Veterinary Medicine
Keywords: Pig BVDV Genome Sequence
CLC: S852.65
Type: Master's thesis
Year: 2003
Downloads: 196
Quote: 2
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Abstract


Designed on the basis of this study, the GeneBank logged 14 bovine viral diarrhea virus (BVDV) genome sequence homology analysis, based on a relatively conservative section 69 of degenerate primers by RT-PCR and nested PCR technique covered compound isolated from suspected swine fever disease of BVDV ZM-95 line 11, both of the genome-wide overlapping cDNA fragments, the amplified fragment was cloned into pGEM-T vector and sequenced, each fragment at least stitching two clones were sequenced the complete genome sequence of the ZM-95 strain, and its system analysis, the results are as follows: 1 genome structure: ZM-95 strains by a 12227bp nucleotide, which accounted for 31.89% A T accounted for 21.93%, G accounted for 26.32%, 19.84% C accounted for. Which comprises the 5 'untranslated region (5'UTR), a large open reading frame (ORF) and the 3' end non-coding region (3'UTR), the ORF encoding approximately 3901 amino acid residues; 5 'UTR of the nucleus The nucleotide sequence length of 337bp, 35bp at least less than the known pestivirus 5'UTR Zone B of the secondary structure has undergone significant structural changes to the base deletion; 3 'UTR nucleotide sequence length of 187bp, with 2-repeat TGTATATA sequence, this sequence repeated in BVDV-I type NADL and Osloss and SD-1 strain 3'UTR also present, this does not exist in the 3'UTR of CSFV and BDV repeat; ZM-95 strain of NS2-3 area is not exogenous sequence insertions. Genome-wide genetic derived relations: Phylogenetic analysis showed that the gene and amino acid level, the ZM-95 strains and BVDV-I-kinship, and BVDV-II, far CSFV and other pestiviruses kinship. Homology analysis shows that the ZM-95 strains and BVDV-I type NADL and Osloss of strain nucleotide homology was 76.0% and 76.3% amino acid homology was 84.5% and 84.0%, respectively; BVDV-II nucleotide and amino acid homology of type 890 were 63.4% and 69.7%, respectively; CSFV strain Alfort, the BDV X818 strains and sickness virus Reindeer-1 H138 strain with the the Giraffe-1 V60 Krefeld strain of nucleotide derived were 60.4%, 61.3%, 60.0%, 57.6% amino acid homology were 64.5%, 65.3%, 66.0%, 63.1%. 3 ZM-95 strain of the structural proteins and non-structural protein N Pro , NS2-3 homology with reference strains: immunogenicity closely related to the structural protein E2 gene of BVDV-I type CV24 strains of the highest homology was 72.5%, with BVDV-I NADL, of Osloss strains homology center, 69.6% and 68.6%, respectively; lower homology with BVDV-type II 890 56.5 %; lowest homology with CSFV C-strain strain, only 52.7% of the non-structural protein NS2-3 gene of BVDV-I SD-1 strain had the highest homology of 79.9%; BVDV-II homology center of type 890, 67.3%; with the CSFV Alfort homology minimum, was 65.3%. Amino acid homology analysis found that the non-structural protein of the pestivirus NS2-3 is a relatively conservative, and lower structural protein E2 conservative. ZM-95 strains had the highest homology with the type I strains of BVDV-gene structure most similar to the ZM-95 strain is a new BVDV-derived from porcine type I strains. In conclusion, this study determined for the first time separated pig BW: JV strains (Ze95) the complete genome sequence and its analysis at the molecular level, to explore the possible evolutionary pathway of this strain of BVDV epidemiological investigation, prevention and control of BVDV and pig BVDV infection and CSFy differential diagnosis provide an important basis.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Basic Veterinary Science > Animal Microbiology ( Veterinary Microbiology, ) > Livestock Virology
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