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Junctional Epithelialization of Dental Implants on Animals in Unloaded Period

Author: LiLeLe
Tutor: LiuLi
School: Zhejiang University
Course: Clinical Stomatology
Keywords: Dental Implants Junctional epithelium Proliferating cell nuclear antigen
CLC: R783
Type: Master's thesis
Year: 2004
Downloads: 93
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Abstract

Background implant implants and implant-supported by the structure and composition of the upper part of a special prosthesis. For conventional denture difficult to get enough support, retention and stability of the patient, implant has obvious advantages. Since the 1960s, the titanium as an ideal implant biomaterials used in clinical. In order to obtain effective long-term implant retention in the body, people continue from planting material type, performance, biological characteristics, planting programs, operating techniques, and postoperative care of all aspects of a lot of research, review and improvement, so that people Implant growing awareness has greatly enhanced the clinical application of implant success rate. Inoue et al found in clinical formed around the implant often blind bags, implant interface can not obtain a valid gingival mucosa closed long junctional epithelium migration into the implant-bone interface, destruction osseointegration, eventually leading to implant loose. Dental implants throughout the oral environment and soft tissue, bone and internal environment, purports to implant in the oral environment without impairing the exercise of functions submucosal bone tissue, it must ensure that the implant - gingival interface healthy and can prevent the destruction of bacteria in the mouth, etc. Environmental factors erode the jaw. Therefore, the success of dental implants is one of the prerequisites to get attached to the surface of the implant neck, oral mucosa biological barrier. Establish and maintain this barrier depends on the oral soft tissue regeneration on implant body attachment and closed neck. Because dental implant surface without cementum and periodontal ligament fibers, junctional epithelium biological barrier seemed Zhejiang University master's degree thesis 20 () 4 are particularly important. If the implant neck can not form or maintain this barrier, bacteria and other proinflammatory cytokines will invade the tissue environment, causing inflammation; and lead to the root end attached to epithelial migration into the implant and bone interface. Once the implant and bone interface has migrated into the epithelial tissue, it will destroy the bone integration, leading to implant loosening the implant to fail. Therefore, the formation and keep wearing the neck of the tooth implant mucosa at pretty epithelial barrier integrity and enhance the protection of bone tissue growing success rate is extremely important [3, \substrate structure attached to the drill-chromium alloy implant surface. later, more scholars Chin, composite resin, ceramic dental implant interface has been studied with the epithelium were found Hemidesmosomes a composite substrate structure and some scholars chemical methods using tissue epithelial tissue implant an interface for further research and found that exists between the implant junctional epithelium layer containing acid mucopolysaccharides and glycoproteins amorphous substance, with the basement membrane staining consistent ingredients which are confirmed the implant neck and the presence of epithelial surface can form interface 16.71. Through our research group has also been found in animal experiments TEM, 15 days after planting pure titanium implant surface epithelial cuff neck structure implant surface epithelial cells visible pseudopodia Hemidesmosomes and base plate structure but had not been planted after the formation of continuous observation of epithelial barrier [\steflik other 101 at the ultrastructural level, proposed the formation of a dynamic process junctional epithelium. He believes that through a combination of epithelial secretory vesicles, the glycosaminoglycan implant surface discharge in the basement membrane through hemidesmosomes a base plate structure so that the implant junctional epithelium adhesion surface epithelial barrier. After planting, Hemidesmosomes a base plate implant a complex is formed in the process of epithelial barrier vital structures. The formation of this structure, the root after the migration of epithelial cells and regeneration functions are closely related. Epithelial barrier formation, epithelial cell adhesion complex and dynamic formation process ultrastructural study is very straightforward. However, in this process, on the epithelial cell regeneration, proliferation was not many studies. Proliferating cell nuclear antigen (prol iferating Cell noelear antigen, PCNA) evaluate cell proliferation can be used as an important indicator of its cell concentration and cell regeneration is closely related. CNA detect the expression level of the corpse can better reflect the status of cells into the cell cycle, cell proliferation represents potential. In this study, using animal models, observed after implantation dental implant neck with epithelial regeneration, root to migrate to form a continuous junctional epithelium histological procedures, and epithelial cells in the process of PCNA expression, and to explore the interface implant an epithelial the formation of epithelial cell proliferation and migration. Zhejiang University master's degree thesis 2004 Objective (1) observed two-stage non-submerged dental implant junctional epithelium formation of non-load process. (2) observed during the formation of junctional epithelium, PCNA expression changes. Research Methods (l) to establish two-stage non-submerged dental implants animal models. (2) HE staining junctional epithelium formation process. (3) ultra-sensitive S-P immunohistochemical PCNA. Results (l) 12d after planting see junctional epithelium begins to form, PCNA expression was strongly positive. (2) 1sd stabilized epithelial cell proliferation, PCNA expression was significantly decreased. (3) 20d formed epithelial attachment similar to natural teeth to maintain low-level expression. Conclusions (1) pure titanium dental implants week period can be formed in a non-load the epithelial attachment similar to natural teeth. (2) during the formation of junctional epithelium, PCNA expression and epithelialization histological changes are closely related.

Junctional Epithelialization of Dental Implants on Animals in Unloaded Period

Abstract

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