Dissertation > Excellent graduate degree dissertation topics show

The tumor sanjie Dan ( containing serum ) induced apoptosis in cervical cancer cell line Hela experimental study

Author: FanLiFeng
Tutor: ZhaoGang
School: Hubei College of Traditional Chinese Medicine
Course: Chinese and Western medicine combined with the basis
Keywords: The tumor sanjie Dan Cervical Cancer HeLa cells Apoptosis Containing serum Acute toxicity test
CLC: R285
Type: Master's thesis
Year: 2004
Downloads: 127
Quote: 0
Read: Download Dissertation

Abstract


Objective: 1. Research on the use of serum pharmacology of anti-tumor drugs tumor sanjie Dan on human cervical carcinoma cell line HeLa cell growth inhibition and induction of apoptosis and cell cycle. 2. Investigate the tumor sanjie Dan possible mechanism of antitumor effect. 3. Dan's understanding of the tumor sanjie acute toxicological effects. Methods: 1. In accordance with the requirements of serum pharmacology preparation containing serum: Dan will be made of the tumor sanjie water decoction orally to rabbits, acquire and rabbit blood serum was separated. 2. Cell culture: The human cervical carcinoma cell line HeLa cells were inoculated with 10% fetal bovine serum in RPMI-1640 medium at 37 ℃ with 5% CO_2 the incubator. 3. Choose serum containing different concentrations of human cervical cancer cell line HeLa cells together 24 hours, using an inverted microscope morphological changes; using MTT reduction assay optical density (OD value), inhibition rate was calculated; using flow cytometry (FCM) detection of tumor-induced apoptosis in HeLa cells Dan sanjie effects as well as effects on the cell cycle. 4. Acute toxicity test in mice by oral gavage administration mode, continuous observation of seven days, the number of animal deaths statistics, access to relevant experimental data and with the modified Karber method for the determination of their LD 50 . Results: 1. HeLa cells were treated with drug-containing rabbit serum for 24 hours, the growth of adherent gradually turned off. Many cell culture medium resuspended, the cells became round, the volume reduced, significant gaps between cells. While the control group and the saline group adherent cells grew well, the adjacent cells fused, showing polygonal, medium no or only see a little cell debris. 2. Serum containing large, medium and small dose group inhibition rates were 56.10%, 48.37%, 34.31%, significantly higher than the control group and the saline group (P <0.05); and the high-dose group was significantly higher than that in the low dose group ( P <0.05). 3. Serum containing large, medium-dose group induced apoptosis percentages were 21.58%, 13.32%, and compared to the control group and the saline group were significantly different (P <0.05); Furthermore, compared to the control group and the saline group, Chinese large, medium-dose group were so G 0 / G 1 phase cells decreased significantly, G 2 / M phase cells increased significantly, S phase cells significantly reduced by different degrees of cell cycle arrest at G 2 / M phase; small dose group makes G 0 / G 1 phase cells significantly increased, G 2 / M phase cells was significantly reduced, S phase cells decreased significantly. 4. The tumor sanjie Dan oral gavage LD 50 is: 10.11 g / kg; 95% confidence limit: 9.41 ~ 10.81g/kg conclusions: 1. The tumor sanjie Dan (containing serum) inhibits human cervical carcinoma cell line HeLa cell growth; 2. The tumor sanjie Dan (containing serum) large, medium-dose group can induce apoptosis in HeLa cells; 3. The tumor sanjie Dan (containing serum) on HeLa cell cycle influential; cell cycle G 2 / M phase arrest may Hubei College 2001 Master of Culture Dan induce tumor sanjie One of the mechanisms of apoptosis in HeLa cells. 4 of tumor sanjie Dan (containing serum) induced apoptosis in HeLa cells with dose and time-dependent: the concentration is too small, too short (2.5%, 12h) insufficient to induce apoptosis; concentration is too high, it is too length (15%, 36h) is easy to cause cell necrosis. 5 of tumor sanjie Dan oral gavage of LDS. As: 10.119/kg; Dan sanjie the tumor has some toxicity.

Related Dissertations

  1. Image-Guided Adaptive Radiation Therapy of Intensity-Modulated Radiation for Cervical Cancer,R737.33
  2. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  3. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  4. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  5. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  6. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  7. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  8. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  9. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  10. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  11. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  12. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  13. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  14. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  15. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  16. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  17. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  18. The Detection and Significance of CK 19 mRNA and MUC1 mRNA in Peripheral Blood in Cervical Cancer Patients.,R737.33
  19. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  20. The Effects of (180F-FDG on the Apoptosis of Eca-109 Esophageal Cancer Cell Line,R735.1
  21. The Signal Mechanism Underlying DON Induced Apoptosis in BHK-21 Cells,S856.9

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology
© 2012 www.DissertationTopic.Net  Mobile