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Objective: ① vitro isolation, amplification dog bone marrow stromal cells (Bone Marrow Stromal Cells BMSCs). ② vitro preparation of dog platelet-rich plasma (Platelet-Rich Plasma, PRP) and observing the biological effects of PRP on BMSCs, evaluation of PRP Biology characteristics. (3) to assess the effect of the BMSCs, PRP and its complex treatment of the dog periodontal bone defect regeneration, tissue engineering methods explore the feasibility for periodontal tissue regeneration. Method the: ① extraction dog iliac bone marrow, gradient centrifugation, amplification of bone marrow stromal cells. The ② extraction Dog venous whole blood twice centrifugal obtain the platelet-rich plasma the PRP. Applications the MTT colorimetric detect PRP effect on the proliferation of BMSCs. Immunohistochemical staining, enzyme kinetics and Coomassie blue method to explore the impact of PRP on the BMSCs alkaline phosphatase activity and total protein content, and examine the impact of PRP on BMSCs differentiation. ③ tricalcium phosphate (Tricalcium Phosphate, TCP) as a carrier, BMSCs, PRP and its complex implantable dog artificial periodontal bone defects. Experimental packet: TCP group, PRP / TCP group, BMSCs / TCP group, BMSCs / PRP / TCP Group, at the same time as a control group to flap surgery. After 6 weeks and 12 weeks, the animals neck the arterial perfusion drawn, conventional fixed, decalcified, dehydrated, embedded in wax dipped, sliced, line Histological observation and measurement. Results: All the experimental group after 6 weeks and 12 weeks have varying degrees of periodontal tissue regeneration, and the control group, only a small amount of periodontal tissue regeneration. When the defect implanted PRP can promote the regeneration of alveolar bone and accelerated bone maturation, but you can not promote the regeneration of cementum. BMSCs alone or in combination with PRP implantation defects apparent periodontal tissue regeneration, including regeneration of alveolar bone, periodontal ligament and cementum, especially after 12 weeks of non-cellular cementum regeneration is characterized by . Conclusion: ① implantation District of BMSCs in vivo microenvironment, can differentiate into periodontal tissues including dental cementum, PRP can accelerate this regeneration, large acellular First Military Medical Lee Master degree the Lun gray cementum formation characteristics. ② P, i.e. when used alone, can promote the regeneration of alveolar bone and accelerate bone formation and promote bone maturation, but it does not promote the regeneration of cementum. ③ using tissue engineering methods, the combination of BMSCs., And P that can accelerate including non-cellular cementum, periodontal tissue regeneration, a new treatment for periodontal tissue regeneration.
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