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Activation of hippocampal nerve cells affected by Ganoderma lucidum polysaccharides on pentylenetetrazol

Author: ZhangJinBo
Tutor: WangShuQiu；ZhangChunBin；MaXiaoRu
School: Jiamusi University
Course: Pathology and Pathophysiology
Keywords: epilepsy pentylenetetrazol garoderma lucidum polysaccharides NF-κB hippocampal neurons immunofluorescence reaction phosphoric acid buffer liquid
CLC: R285.5
Type: Master's thesis
Year: 2007
Downloads: 110
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Abstract


Objective: Hippocampal neurons of neonatal Wistar mice is cultured to observe the growth state and prepare model of cell epilepsy. In order to study the influence of garoderma lucidum polysaccharides to NF-κB which expressed in epilepsy model cell. Methord: 30 neonatal Wistar mice were killed to get hippocampal neurons which were cultured for seven day. hippocampal neurons were divided into 3 groups at random: Garoderma lucidum polysaccharides,epilepsy model group and blank group. immunofluorescence reaction was took in different time:0.5 hour、1 hour、6 hours、12 hours. After closing by serum NF-kB/p65 antibody was added. Then it was washed by phosphoric acid buffer liquid before adding FITC-IgG antibody to hatch. Finally it was dyed by propidium and observed with the fluorescence microscope. NF-κB/p65 subunit are marked green by FITC-IgG antibody. cell nucleus are marked red by propidium. applying the immunity organization chemistry to examine each variety of related index sign. Results: culturing hippocampal neurons of neonatal mice are successfully. they grow at the seventh day to prepare modle of cell epilepsy. It is proved that the expression of NF-κB in nuclear are more than garoderma lucidum polysaccharides and blank contrast group. It hint that hippocampal neurons have been active. the expression of NF-κB in garoderma lucidum polysaccharides are less than in epilepsy model group. Conclusion: According to the results, the method of cultured hippocampal neurons which use culture medium and medicine is available, hippocampal neurons grow well,.pure hippocampal neurons add up to 90%.providing better model study experiment of correlative hippocampal neurons . it is testified that active NF-κB in nuclear is relate to epilepsy pathogenesis. The expression of NF-κB in nuclear is sign of active hippocampal neurons. garoderma lucidum polysaccharides can effectively reduce cell [Ca2+] overload of the neuron cell effectively, indirectly inhibit the expression of NF-kB in nuclear to reduce excitability of neurons. So garoderma lucidum polysaccharides is an assistant medicine to cure epilepsy,it can be very useful in clinic application.

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology
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