Dissertation > Excellent graduate degree dissertation topics show

Study of Static Magnetic Fields (SMF) with Cis-platin(DDP) on Human Leukemia Cells(K562)

Author: LiuJianQiang
Tutor: QiHao
School: Shaanxi Normal University
Course: Cell Biology
Keywords: Static magnetic field MTT assay An atomic force microscope Single Cell Gel Electrophoresis Cell cycle DNA cisplatin Western blot Cell
CLC: R733.7
Type: Master's thesis
Year: 2007
Downloads: 72
Quote: 1
Read: Download Dissertation

Abstract


Objective: To study static magnetic field combined with cisplatin on K562 Cells mechanism; K562 cells Cyclin B1 expression changes by immunoblotting (western blotting) before and after the processing of the detected magnetic field. Method: 1. MTT assay cisplatin the joint magnetic field on K562 cells (human erythroleukemia cell) activity changes. 2. Magnetic field combined with cisplatin role in K562 cells by optical microscope, the atomic force microscope changes in cell morphology; analyzed by flow cytometry to detect cell cycle distribution changes; detect DNA damage in the single cell gel electrophoresis (SCGE). 3. Synchronization phase cycle of K562 cells detected by western blotting trend Cyclin B1; 4. K562 cells were treated after the magnetic field changes with western blotting detection Cyclin B1 protein content. Results: 1. The K562 cell vaccination cell density of 1 × 10 ~ 5 cells / mL, MTT test results show that the magnetic field combined with the 10μg/mL cisplatin treatment K562 cells 12h after cell activity was significantly inhibited. 2. The magnetic field combined with 10μg/mL K562 cells were treated with cisplatin 12h after the optical cell morphology was observed under a microscope found that the magnetic field combined with cisplatin group more cell debris. The atomic force microscope found that the group of cell surface magnetic field combined with cisplatin larger holes. Flow cytometry cell cycle distribution found that the magnetic field may be the cell cycle arrest at the G2 / M phase; cisplatin cells in S phase arrest; cells 12h after cisplatin combined magnetic field treatment, most of the cells were blocked in S period. The SCGE detection results show that DNA damage can increase the magnetic field synergy Cisplatin K562 cells. The atomic force microscope magnetic field with cisplatin common K562 cells, changes in the structure of the DNA, found that the combined treatment group DNA cross-linking and fracture. 3. Cyclin B1 protein western blotting detection cycle, the results show that the K562 cell Cyclin B1 expression started from the beginning of the S phase, and then gradually increased to the highest point in the G2 / M phase into M phase decreased rapidly. 4. The magnetic field is treated separately K562 cells 12h, 24h, 36h, with western blotting detection Cyclin B1 content, found that magnetic treatment group compared with the control group, Cyclin B1 protein expression levels did not significantly change. Conclusions: 1) magnetic field combined with anticancer drugs (cisplatin) of K562 cells under certain conditions, a synergistic killing effect, mainly in the impaired cell morphology, cell cycle distribution change with cell DNA damage increased. 2) the magnetic field, respectively, in K562 cells 12h, 24h, 36h, western blotting to detect changes in the content of cell Cyclin B1 magnetic treatment group compared with no significant change in the expression of Cyclin B1 and control.

Related Dissertations

  1. Numerical Analysis of Flow Process in a Molten Carbonate Fuel Cell,TM911.4
  2. Fabrication of Membrane Electrode Assembly for PEMFC,TM911.4
  3. Research on the Effects of Protection and Renovation of Apple Polyphenols on UVB-Induced HepG2 Cell Injury,S661.1
  4. Research and Realization on Automatic Generation of Computing Engine for Myocardial Cell Electrophysiological Simulation,R319
  5. The Design and Accomplishment of Simulition Platform for Voltage Clamp Experiment Based on Cell Electrophysiological Model,R318.0
  6. Development of the Locomotory Cell Test and Contorl Module Based on VXIbus,TP274
  7. Study of Oridonin on SGC-7901 Cell Proliferation by Inhibitting Cell Cycle Proteins,R285
  8. Functial Characterization of Transcription Factor Moswi6 in Magnaporthe Oryzae,S435.111.4
  9. Expression of Prolyl Isomerase Pin1 in Osteosarcoma and the Effect of Regulation on Cell Cycle,R738.1
  10. Comparison on Immunoprotective Effect of Bacteriophages of Vibrio Alginolyticus and Vaccines of Bacterial Ghost,S945
  11. The Elective Toxic Effect of One Component Separated from Cobra Snake Venom on the Neuronal Cell and Its Possible Mechanisms,Q51
  12. The Study in the Influence of the Cell Phone on the Moral Values of the College Students and the Countermeasure,G641
  13. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  14. Research on the Methods of Nasopharyngeal and Gastric Cancer Detection Based on Surface-enhanced Raman Spectroscopy,R739.63
  15. Pt -based catalysts of direct methanol fuel cells,TM911.4
  16. Studies on Dynamic Regularity of Antibody Against G. Anatis and Development of Hybridoma Cell Lines Secreting Monoclonal Antibodies Against G. Anatis Lps,S858.32
  17. Cryopreservation and Damage Research of Sperm of Cobia (Rachycentron Canadum) and Crimson Snapper (Lutjanus Erythopterus),S961.2
  18. Development of Hybridoma Cell Lines Secreting Monoclonal Antibodies Against Lps Antigen of B. Melitensis,R392
  19. miRNA-23a into the regulation of muscle differentiation in C2C12 cells,Q952.5
  20. Construction and Identification of Recombinant Lentivirus Expressing shRNA Targeting to ORF1 and ORF7 of Porcine Reproductive and Respiratory Syndrome Virus Genomes,S852.65
  21. The Methode of High Density Cells’ Tracking Based on Topological Constraint Combined with Hungarian Algorithm,Q25

CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia
© 2012 www.DissertationTopic.Net  Mobile