Dissertation > Excellent graduate degree dissertation topics show

Preparation of β-c from Dunaliella Salina Submicron Emulsion and Study on Its Cytoactive

Author: TangQiLing
Tutor: WangChunBo
School: Qingdao University
Course: Pharmacology
Keywords: The D. salina beta - carotene Submicroemulsion 9 - cis - beta - carotene Apoptosis
CLC: R94
Type: Master's thesis
Year: 2007
Downloads: 239
Quote: 0
Read: Download Dissertation

Abstract


Purpose extracted from Dunaliella beta - carotene ( beta - C ) ; establishment of cis , trans- beta - C isomers method for the determination ; preparation of the D. salina beta - C submicroemulsion study its the anti - 60 < / sup > Co radiation effect . Method of a confined extraction extraction process Dunaliella beta - C ; HPLC method for the determination of Dunaliella all-trans- beta- C , 9 - cis - beta - C content ; using orthogonal test method to determine the Dunaliella beta - C submicroscopic the best formulation and preparation process of the milk ; established 60 Co radiation ( strength 3Gy ) damage pathological model of mouse thymus lymphocytes (in vitro) , divided into six groups : control group , 60 < / sup > Co model group , 60 < / sup > Co 0.64μg · mL -1 D. salina beta- C group , 60 Co 0.32μg · the mL -1 D. salina beta- C group , 60 < / sup > Co 0.16μg · mL -1 D. salina beta- C group , 60 Co 0.1mg · mL -1 VitE group ; thymus lymphocytes of mice were observed by transmission electron microscopy ultrastructural changes ; MTT method and Brdu method determination of the murine thymocytes activity and proliferation ; agarose gel electrophoresis apoptosis ; Western blot determination of p38 protein expression . Dunaliella extract total BC content of 226.77mg · g -1 , where 9 - cis- beta - C content of 84.60mg · g -1 < / sup > ; submicron drops of breast milk round morphology , particle size distribution , a unimodal distribution , volume weight diameter of 230 ± 20 nm , zeta potential of -59.29 ± 6.10mv ; optical , thermal stability ; can inhibit salt algae beta - C < sup > 60 < / sup > Co radiation -induced thymic lymphocyte apoptosis significantly increased cell activity , a significant increase in the proliferation of cells , suppression 60 < / sup > Co radiation murine thymocytes in P38 protein phosphorylation , statistically significant difference ( P < 0.05 ) . Conclusion scientific and advanced extraction process and a method for the determination ; salt algae beta - C sub - microemulsion process is reasonable , stable ; salt algae beta - C has anti- 60 Co radiation effect , and a dose-dependent in the dose range of 0.16 to 0.64μg/mL .

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  3. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  4. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  5. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  6. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  7. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  8. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  9. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  10. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  11. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  12. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  13. The Impact of Histone Deacetylase Inhibitor Trichostatin a on Proliferation and Apoptosis of Porcine Granulosa Cells,S828
  14. Antitumor Effects of Elemene on the Proliferation Inhibition and Apoptosis Induction in Ascites Hepatoma Cell Line H22,R735.7
  15. Expression and Their Significance of Apoptosis Regulatory Factors Survivin、XIAP and c-Jun、NF-κB in Epithelial Neoplasm of Ovary,R737.31
  16. Therapeutic Effect and Mechanisms of Derived Mesenchymal Stem Cells in Intracerebral Hemorrhage Rat,R743.34
  17. Inhibitory Role of Plasmodium Yoelii Circumsporozoite Protein on the Proliferation and Survial of Tumor Cell,R730.5
  18. Effects of Different Immunization on the Development of Immune Organs,cell Proliferation and Expression of Apoptosis-relatived Gene in Chicken,S858.31
  19. Induction of Apoptosis by d-limonene is Mediated by Mitochondrial Death Pathway in Human Leukemia Cells,R733.7
  20. Effects on Recombinant Human Interleukin-10 of IL-2, IFN-γ, GCs, T Cell Apoptosis in Transplantation of the Rabbit Skin,R392
  21. Edwardsiella Tarda Infection on Andrias Davidianus and the Protection of Dead Vaccine at Primary Immune,R392.1

CLC: > Medicine, health > Pharmacy > Pharmacy
© 2012 www.DissertationTopic.Net  Mobile