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Plant somatic embryogenesis is an important manifestation of the totipotent plant cells under the appropriate culture conditions to high frequency regeneration of complete plants, is a plant genetic improvement receptor system. Somatic embryogenesis generally undergo a dedifferentiation process, namely the vegetative growth of the body cells into embryonic cells of the reproductive growth. Many somatic embryogenesis related genes in zygotic embryos similar expression, indicates somatic embryogenesis the zygotic embryogenesis there may be similar gene expression and regulation. Therefore, to study the regulation of gene expression of somatic embryogenesis molecular mechanism of the genesis and development of the understanding of plant cell differentiation and zygotic embryos has important theoretical significance. Full access to domestic and foreign plants zygotic embryos and somatic embryogenesis genetic data on the basis of this study initially identified a number of candidate genes, such as AtSERK1 PIN1, PIN7, MP, LEC2, WUS, AGL15 and BBM, etc., but if embryogenesis sustained overexpression of related genes in the transformed cells, often leading to transformed cells can not differentiate or teratogenic seedlings impact of transgenic genetic transformation efficiency and genetic improvement effect of transgenic plants. The plasmid vector PINDEX3 is a dexamethasone-induced expression and regulation system, very strict regulation of the expression of the exogenous gene at the transcriptional level, has been successfully applied to Arabidopsis, tobacco, rice, and other plants. Therefore, we will be amplified by the WUS and AGL15 gene connected to PINDEX3, build out their dexamethasone-induced expression and regulation system, the WUS and AGL15 genes in transformed cells, limited quantitative expression regulation inducer, and preliminary study their role in the regeneration of tobacco, the results are as follows: 1 amplified from Arabidopsis WUS and AGL15 genes using RT-PCR technology. Sequence analysis showed that the WUS gene amplification sequences have been reported (NM 1 27349) exactly the AGL15 gene amplification has been reported in the sequence (NM 1 21382) the occurrence of one mutation, the open reading frame of 563 base reported A mutated into G encoding amino acids from aspartic acid to glycine. 2 Construction of the plant inducible expression vector pIWUS (WUS gene) and pIAGL15 (AGL15 genes), and import them into Agrobacterium GV3101 leaf disk hygromycin-resistant tobacco plants, turn WUS gene detected by PCR The AGL15 genes positive plants 12 lines (A total of 86 lines) and turn positive plants six lines (A total of 34 lines). 3, transgenic plants (WUS and AGL15) did not exhibit abnormal phenotype change in the circumstances of non-inducing agent plus dexamethasone.
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