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Advances in Molecular Mechanism and Prolonging Life of Ginseng

Author: BaiLiHua
Tutor: LiShuGuang；CaiZhiMing；ZhangXinWen
School: Tongji University
Course: Nutrition and Food Hygiene
Keywords: Ginseng Ginsenosides Edible oil Antioxidant Survival Test Drosophila melanogaster HPLC Antioxidant enzymes RT-PCR Extraction Process
CLC: R285
Type: Master's thesis
Year: 2006
Downloads: 248
Quote: 2
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Abstract


Purpose: (1) understanding of ginseng ginseng ginseng Edible Oil from the Drosophila life in its the lipid peroxidation antagonism; (3) explore the Drosophila Drosophila melanogaster life and its lipid peroxidation; (2) to understand drosophila and its underlying lipid peroxidation in the molecular mechanisms; (4) Compare ultrasonic extraction, soaking method and Soxhlet extraction method in three different ways and different extraction time on the similarities and the differences in the effects of ginsenosides extracted. Method: (1) use of the the Drosophila survival test and spectrophotometry ginseng impact on the the Drosophila life of its in vivo antioxidant system, to explore the relationship between Drosophila aging; (2) use of a the Drosophila survival test and spectrophotometry ginseng cooking the remaining oil to the Drosophila lipid peroxidation antagonism, to explore the relationship between Drosophila aging; (3) by RNA extraction, RT-PCR and other molecular biology methods to further explore ginseng of fruit flies antioxidant enzymes (SOD, CAT) gene mRNA expression levels change; (4) using a Shimadzu ODS C18 column with a mobile phase of acetonitrile - water gradient elution, detection wavelength was set at 203nm, Health and drying parameters for quantitative analysis to observe the different extraction methods and extraction time on the content of ginsenosides. Results: ① The average life expectancy of each dose group of male and female fruit flies (MLS) and the average maximum life span (MMLS) were higher concentrations of 0.30% and 0.90% of female flies MLS extended 3.39d and 3.73 d ( P <0.05); the male flies MLS respectively extend 2.51d and 2.70d (P <0.01); 0.03% to 0.90% concentrations of male flies MMLS extended 2.20d to 5.40d (P <0.05, P <0.01), 0.30% and 0.90% concentrations of female flies MMLS extended 3.80d ~ 4.42d (P <0.01). Each group of male and female flies fed sample section 10D, 20D when the increase in total SOD activity than the control group (P <0.05); 0.30% ginseng group of female flies and 0.90% the ginseng group of male flies fed samples 10d and 20d The total SOD activity when compared with the 0.10% increase in the ginseng group (P <0.05), and Drosophila total SOD activity of ginseng content in the medium increments. Each group of male and female fruit flies in the feeding of the sample section 10d, 20d when the MDA content than the control group (P <0.05) decrease; 0.30% ginseng group of female flies and 0.90% the ginseng group of male flies fed samples 10d and 20d of MDA content of ginseng group compared with 0.10% decrease (P <0.05), 0.30% ginseng group males and 0.90% ginseng group of female flies in the the feeding sample first 10d MDA content compared with 0.10% ginseng group decreased (P <0.05) and Drosophila MDA content in the body decreases with ginseng content in the medium. ② fed high concentrations of ginseng and cooking oil group left male and female fruit flies MLS MMLS were higher than only feeding Edible oil group Drosophila fruit flies but lower than that of the control group (P <0.05), male and female fruit flies MLS cooking residual oil group extended 2.66d to 7.41d (P <0.01); feeding of ginseng and cooking oil left Drosophila SOD activity level is higher than only feeding Edible Oil from Drosophila fruit flies below the control group With ginseng content in the medium increments; the MDA content below only to feed cooking oil left Drosophila but higher than that of Drosophila and with ginseng content in the medium Descending. ③ certain dose of ginseng can Drosophila superoxide dismutase mRNA expression levels of antioxidant enzymes catalase. The The ④ ginseng saponin Rb1, Re and Rg1 injection volume of 0.5003μg to 7.5045μg, of 0.5009μg to 7.5135μg 0.5012μg ~ 7.518μg range of its content and the peak area was a good linear relationship. Conclusion: ① ginseng can enhance the activity of antioxidant enzymes SOD in Drosophila melanogaster, to reduce the generation of lipid peroxidation products MDA, which may extend the life of fruit flies; ② in a certain concentration range ginseng can be part of the antagonistic Edible oil causes the Drosophila lipid peroxidation, cooking oil left in Drosophila Melanogaster lipid peroxidation damage a protective effect; (3) ginseng can improve the Drosophila brain tissue cells CAT, SOD gene expression, which may play anti- oxidation anti-aging effect; ④ Soxhlet extraction method is better than the ultrasonic method is better than the soaking method.

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