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Differentation and Apoptosis Induiced by Tanshinone ⅡA Combined with Arsenic Trioxide in NB4 Cells

Author: ZhangGe
Tutor: YangYiMing
School: Sichuan University
Course: Internal Medicine
Keywords: Tanshinone Ⅱ A Arsenic trioxide Apoptosis Cell differentiation NB4 cell lines
CLC: R733.71
Type: Master's thesis
Year: 2007
Downloads: 77
Quote: 0
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Abstract


Objective To study tanshinone Ⅱ A (Tan Ⅱ A) and arsenic trioxide (As 2 O 3 ) joint use of NB4 cell differentiation, and apoptosis in the presence or absence of synergy. The method uses the combined effects of Tan Ⅱ A 1.0μg/ml respectively NB4 cells. Observe the different time periods of drug action, the number of living cells, growth curve and calculate the experimental days of growth inhibition rate; Giemsa staining, transmission electron microscopy morphological changes were observed; flow cytometry Annexin Ⅴ Determination of the rate of apoptosis, and cell cycle and apoptosis analysis. Results 1. Tan Ⅱ A with As 2 O 3 joint NB4 cells synergistic inhibitory effect on the growth of this inhibition with the duration of action and joint As 2 O < sub> 3 is increased by the increase of the concentration. The NB4 cells 5 days 1.0μg/mL Tan Ⅱ A role inhibition rate was (45.01 ± 4.82)%, respectively, with 0.125μmol / L, 0.25μmol / L, and 0.5μmol / L As 2 O < sub> 3 combined effects of five days of NB4 cell growth inhibition rates were (82.05 ± 3.04)%, (94.06 ± 0.95)% and (95.86 ± 2.08)%, are significantly stronger than its separate role of NB4 Cell growth inhibition rate (p <0.01). 2. Tan Ⅱ A with As 2 O 3 United NB4 cells induced apoptosis synergy. 1.0μg/mL Tan Ⅱ A 0.5μmol / L, and 1.0μmol / L As 2 O 3 joint NB4 cells one day NB4 cell apoptosis rates were (4.20 ± 0.36)% and (20.17 ± 2.15)% than the corresponding single drug group of pro-apoptotic effects [(1.67 ± 0.67)% and (9.43 ± 0.51)%] was significantly enhanced (p <0.05), Tan Ⅱ A and 0.5μmol / LAs 2 O 3 joint proapoptotic synergies continuing role 72h. 3. Tan Ⅱ A with As 2 O 3 United NB4 cell proliferation index (PI) synergistic inhibitory effect in the G0/G1 phase cells increased; decrease in S phase cells. The 1.0μg/mL Tan Ⅱ A and 0.125μmol/L0.25μmol/L and 0.5μmol / L As 2 O 3 combined effects of NB4 cells 3 days PI, respectively ( 33.40 ± 1.01)%, (33.73 ± 1.72)% and (32.93 ± 4.32)%, PI values ??(p <0.05) were lower than the corresponding single drug. 4. Tan Ⅱ A with As 2 O 3 United NB4 cells induced differentiation without enhancement. Conclusion: 1. Tan Ⅱ A Joint As 2 O 3 NB4 cells induced apoptosis obvious synergies. Such synergies and joint use As 2 O 3 concentration is closely related; 2. Tan Ⅱ A Joint As 2 O 3 NB4 cells induced to differentiate not shown obvious synergies.

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CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia > Acute leukemia
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