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Chemiluminescence analysis with background interference from light scattering effects and high sensitivity, simple equipment, easy to operate, with a wide range of applications in the determination of biological molecules. In recent years, nanomaterials-based liquid chemiluminescent analysis aroused widespread concern. This thesis is based on gold nanoparticles sol excellent catalytic performance and luminescence properties of CdS Nanocrystals gold nanoparticle sol on luminol - Over the the liquid chemiluminescent catalytic hydrogen peroxide chemiluminescence system performance and CdS Nanocrystals participation process , and accordingly to establish some common biological molecules measured chemiluminescence system specific summarized as follows; (1) based on gold nanoparticle-catalyzed luminol - hydrogen peroxide chemiluminescence determination of estrogen. The gold nanoparticles sol strongly enhanced luminol - hydrogen peroxide chemiluminescence, and natural estrogen can significantly suppress the gold sol catalytic Luminol - hydrogen peroxide chemiluminescence system. Accordingly, we have established a chemiluminescence method for the determination of natural estrogens (estrone, estradiol and estriol). Optimization of experimental conditions, the determination of the linear range of estrone, estradiol and estriol were 0.07 ~ 7.0μmol / L, 0.04 ~ 10μmol / L and 0.1 ~ 10μmol / L. The detection limits were 3.2 nmol / L, 7.7 nmol / L and 49 nmol / L, the relative standard deviation of 2.9%, 2.6% and 1.8%. This method is used estrogen hormone determination of the total amount of the urine samples of pregnant women, with satisfactory results. (2) based on gold nanoparticle-catalyzed luminol - hydrogen peroxide chemiluminescence determination of some thiol compounds. Glutathione, L-cysteine ??and 6 - mercaptopurine strongly suppress gold nanoparticle-catalyzed luminol - hydrogen peroxide chemiluminescence. Based on this, we have established the determination of glutathione, L-cysteine ??and 6 - mercaptopurine, the chemical luminescence method. Under optimal experimental conditions, glutathione, L-cysteine, and 6 - mercaptopurine linear range of 7.0 × 10 -7 to 1.0 × 10 -5 mol / L, 1.0 × 10 -9 to of 7.0 × -6 mol / L and of 4.0 × -8 ~~ 7.0 × 10 -6 mol / L; detection limits were of 1.1 × -8 mol / L, of 2.9 × -10 mol / L and 1.1 × 10 -9 mol / L; relative standard deviation was 1.4%, 2.1% and 1.8%, respectively. (3) based on gold nanoparticle-catalyzed luminol - hydrogen peroxide chemiluminescence system Determination of quinolon by derivatives. Carbostyril derivatives can enhance gold nanoparticle catalyzed luminol - hydrogen peroxide chemiluminescence. Induction of gold oxidation state of the carbostyril derivative may be energy transfer to the 3 - amino-phthalates, and thus increase the excited state of the 3 - amino phthalates quantities, causing chemical emission intensity increases. Based on this, we have established the Determination of the Six the carbostyril derivatives chemiluminescence method. Norfloxacin, ciprofloxacin, lomefloxacin, fleroxacin, ofloxacin and levofloxacin linear range were 0.08 ~ 1.28μg/mL, 0.013 ~ 1.32μg/mL, 0.014 ~ 1.4μg/mL, 0.029 ~ 1.46μg/mL, 0.02 ~ 1.0μg/mL 0.01 ~~ 1.44μg/mL; detection limit of 3.2 ng / mL, 9.5 ng / mL, 7.0ng/mL, 9.0 ng / mL and 8.0 ng / mL and 8.0 ng / mL; The relative standard the deviations were 4.3,1.5,1.9,1.3,1.6 and 2.1%. This method is used for the detection of real samples with satisfactory results. (4) CdS Nanocrystals induced chemiluminescence system and its analytical applications in alkaline solution peroxide hydrogen direct oxidation of thioglycolic acid coated CdS Nanocrystals produce chemiluminescence phenomenon. We found that the particle size of the chemiluminescence intensity CdS nanocrystalline correlation. By means of UV-visible spectroscopy, chemical luminescence spectroscopy, photoluminescence spectroscopy and TEM, we propose a mechanism of CdS Nanocrystals. In addition, we also found that some metal ions and small biological molecules can be strongly inhibited by cadmium sulfide - hydrogen peroxide chemiluminescence, pursuant to which we have established a new method of chemiluminescence determination of biological molecules and metal ions.
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