Dissertation > Excellent graduate degree dissertation topics show

The Effects of Thrombospondin 1 in Transforming Growth Factorβ1 Induce Rat Cardiac Fibroblasts

Author: ChenLing
Tutor: HuJianXin
School: Nanchang University
Course: Internal Medicine
Keywords: Thrombospondin Vascular endothelial growth factor Antisense oligonucleotides Rat cardiac fibroblasts
CLC: R542.21
Type: Master's thesis
Year: 2007
Downloads: 74
Quote: 0
Read: Download Dissertation

Abstract


Objective: To investigate thrombospondin 1 (TSP-1) and vascular endothelial growth factor (VEGF) in rats induced by transforming growth factor-β1 (TGF-β1) myocardial fibroblast proliferation and collagen synthesis process, TSP- 1 and VEGF relations provide a theoretical basis for the prevention and treatment of myocardial fibrosis. Method: digestion differential paste the wall method of primary cultured myocardial ,3-4 cells into fiber cells used in the experiment, the cell morphology was observed under an inverted microscope, anti-fibronectin protein antibody staining cells were identified. The experiment was divided into five groups: (1) control group, did not add any inducer cultured for 24 hours. ② of TGF-β1 in a dose-dependent group, were added to 10ng/ml, 20ng/ml, 30ng/mlTGF-β1 cultured for 24 hours. ③ TGF-β1 time-dependent group, join the TGF-β1 were cultured for 12, 24 and 48 hours. ④ antisense oligonucleotide group, added TGF-β1 and TSP-1 antisense oligonucleotides and cultured for 24 hours. The the ⑤ sense oligonucleotide Group added TGF-β1 and TSP-1 sense oligonucleotide after 24 hours of incubation. The growth of the number of Hydroxyproline Determination of collagen content were detected by MTT cardiac fibroblasts (CFs). By semi-quantitative RT-PCR analysis of VEGFmRNA in TSP-1 mRNA expression. The VEGFmRNA relative expression and the TSP-1mRNA relative expression level of correlation analysis. Results: 1, myocardial fibroblast proliferation and collagen: group of TGF-β1-induced myocardial into fibroblasts the MTT reaction A value and collagen content were significantly higher than those in the control group (P lt; 0.01) compared with control. The MTT reaction of TSP-1 antisense oligonucleotide TGF-β1 group A value and collagen synthesis magnitude were significantly lower than the TGF-β1 group (P lt; 0.01). No significant difference between the TSP-1 sense oligonucleotide group with TGF-β1 group (P gt; 0.05). 2 mRNA expression: (1) VEGFmRNA expression: ① 10ng/mlTGF-β1 group and the culture of the 12-hour group VEGFmRNA relative expression compared to the control group significantly increased (P lt; 0.01) ② from 20,30 ng/mlTGF-β1 group and 24 hours, 48 ??hours the group VEGFmRNA the relative expression than that of the control group was significantly lower (P lt; 0.01). ③ TSP-1 antisense oligonucleotide group than 20ng/mlTGF-β1 group of VEGFmRNA the relative expression level was significantly higher (P lt; 0.01). ④ TSP-1 the sense oligonucleotide Group compared with 20ng/mlTGF-β1 groups of VEGFmRNA in relative expression levels, no significant changes (P gt; 0.05). (2) TSP-1mRNA expression the: ① 10ng/mlTGF-β1 group and culture of TSP-1mRNA in 12 hours group relative expression than that of the control group had no significant difference (P gt; 0.05). ② groups 20,30 ng/mlTGF-β1 and 24 hours and 48 hours groups of TSP-1mRNA the relative expression was significantly higher than that of the control group (P lt; 0.01). The ③ TSP-1 antisense oligonucleotide Group 20ng/ml TGF-β1 compare the TSP-1mRNA relative expression levels significantly decreased (P lt; 0.01). The ④ TSP-1 sense oligonucleotide Group over 20ng/mlTGF-β1 group relative expression level of TSP-1mRNA no significant change (P gt; 0.05). (3) Correlation analysis: correlation analysis showed that expression of the amount of time and concentration-dependent group VEGFmRNA of TGF-β1 and TSP-1mRNA r values ??of -0.537 and -0.714 (P lt; 0.01). VEGF and TSP-1 expression was negatively correlated. Conclusion: TGF-β1-induced rat cardiac fibroblast proliferation and collagen synthesis process elevated TSP-1 expression is induced by TGF-β1. 2.TSP-1 expression is inhibited VEGF expression. 3.TSP-1 antisense oligonucleotides can inhibit myocardial TGF-β1-induced fibroblast proliferation and collagen synthesis. 4.TSP-1 antisense oligonucleotide may have the role against myocardial fibrosis.

Related Dissertations

  1. The Expression of MMP-2、 VEGF and Ki-67 and Human Glioma Grade,R739.41
  2. The Research on Treatment of Lentivirus-mediated hVEGF-165 Gene Transferring Mesenchymal Stem Cells on Rat Hind Limb Ischemia,R329
  3. BMP2 and VEGF165 Genes Transfected Rat Bone Marrow Mesechymal Stem Cells Simultaneity Expression,R329
  4. Expression of TNF-α, MMP-9 and VEGF in BALF of Rats with Emphysema and Their Mutual Relationship,R563.3
  5. Influence and Significance of MMP-2 and VEGF of Hemangioma Endothelial Cells in Vitro Culture by Propranolol,R739.5
  6. The Investigation of Relationship between Therapeutic Effect and Prognosis and Changes of Peripheral Blood Vascular Endothelial Growth Factor and Metallothionein in Patients with Advanced Lung Cancer,R734.2
  7. Study on the Correlation btween Serum, Aqueous Humor VEGF, IL-6 Levels and the Neovascular Glaucoma,R775
  8. The Analysis of the Growth Factor Related with the Secondary Glaucoma in the Anterior Champer of Silicone Oil-filled Eyes,R779.6
  9. Vascular endothelial growth factor on endothelial cells concentration of magnesium ions,R965
  10. Experimental Studies on Therapy of Lung Cancer by VEGF-C siRNA Mediated by Liposomes,R734.2
  11. Experimental Study of the Relationship between Pulmonary Metastasis in Osteosarcomas and Vascular Endothelial Growth Factor Expression and Microvessel Density,R738.1
  12. The Effect of IL-17 on VEGF Expression in Human Nsclc Cell in Vitro,R734.2
  13. Propranolol for Haemangiomas-insight into VEGF/ERK Signaling Transduction in HUVEC-12 Cells,R965
  14. Effect of Candesartan on Expression of VEGF and MCP-1 in Rat Retina with Diabetetes Mellitus,R774.1
  15. Correlation between CT Perfusion Imaging in Nonsmall-cell Lung Cancer and VEGF、MVD、VEGF-C、LVD,R734.2
  16. Expression of Survivin、FHIT、VEGF-C in Gastric Adenocarcinoma and the Correlation Among the Three Factors,R735.2
  17. Relaionship among Expression of ESM-1, VEGF-D, Lymphangiogenesis and Lymph Node Metastasis in Human Invasive Lobular Breast Cancer,R737.9
  18. The Effect of siRNA Targeting MIF on the Growth of Colorectal Neoplasms Xenografts and the Life Quality of Tumor-bearing Mice,R735.34
  19. Experimental Study of the Chemotaxis Effect of Vegf in the Treatment of Cerebral Ischemia with Bone Marrow Stromal Cells,R743.3
  20. The Roles and Mechanism of HIF-2α in Pathogenesis of Ischemic/Hypoxic Acute Kidney Injury,R692
  21. Relation between VEGF and Vascular Positive Remodeling after Stent-implantation and the Effect on the Expression of VEGF and MAC by Different Stents,R541.4

CLC: > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Heart disease > Myocardial diseases > Myocarditis
© 2012 www.DissertationTopic.Net  Mobile