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Hunan swine genetic diversity of Enterococcus faecalis

Author: LinRongGao
Tutor: LiManXiang
School: Hunan Agricultural University
Course: Preventive Veterinary Medicine
Keywords: Enterococcus faecalis AFLP Phenotype Genotype
CLC: S852.611
Type: Master's thesis
Year: 2011
Downloads: 29
Quote: 0
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Abstract


Enterococcus faecalis is a Gram-positive cocci , but had a normal person ordinarily resident bacteria and animal digestive tract has been ignored , and later became the hospital cross-infection due to the culprit , as well as its widespread resistance to antibiotics in human public health field concern. In recent years, reports of animals infected with Enterococcus faecalis start to appear , but in the field of animal medicine or not enough attention in terms of research and data accumulation is not much . This experiment diseased swine from clinical isolates of Enterococcus faecalis , infection of the popular analysis and the use of traditional techniques for resistant strains and biochemical characteristics of study to understand the situation in Hunan Province and strain characteristics of strain infection , while the use of AFLP technique , 16S rDNA sequencing and detection of virulence genes to further understand the genetic diversity of strains and phylogenetic relationships . Experiments show that : Hunan isolates ( 42 ) separating the highest rate in the lungs ( 69.04% ) , autumn and winter are more likely to lead to strain the situation , most of the city in Hunan pigs infected with Enterococcus faecalis exist . Hunan isolates carrying the virulence genes very different number and type of different virulence gene detection rate varies according to the number of virulence genes and species carrying obtain 14 kinds of different virulence distinguish genotypes . Most isolates with multiple drug resistance, generally are resistant to tetracycline , resistant strains behave quite different, vancomycin and penicillin G probability of emergence of resistance less likely these two drugs is more suitable for Hunan Enterococcus faecalis infections , 42 Enterococcus faecalis and biochemical characteristics in line with \Experiments established AFLP analysis of Enterococcus faecalis reaction system suitable for general laboratory operations , the use of AFLP patterns can be separated in Hunan variety genotype isolates , some strains of these genotypes may reflect differences between and infer some strains may belong to the same clone . AFLP fingerprints obtained by 16S rDNA sequencing can be more than a clear description of Enterococcus faecalis intraspecific differences between former can better understand the genetic diversity of Enterococcus faecalis and ecological diversity .

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Basic Veterinary Science > Animal Microbiology ( Veterinary Microbiology, ) > Pathogenic bacteria > Cocci
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