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DBD Plasma Applications in Biomedicine

Author: WangXiaoLin
Tutor: FengKeCheng
School: Changchun University of Science and Technology
Course: Physical Electronics
Keywords: DBD Plasma Teflon Hydrophilic angle Human lung cancer cells (A549) Neutral red uptake method
CLC: O539
Type: Master's thesis
Year: 2011
Downloads: 36
Quote: 0
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Abstract


Dielectric barrier discharge (DBD) low temperature plasma generated in biomedical applications is a hot topic in interdisciplinary This thesis is based on the principle of dielectric barrier discharge , use a new type of atmospheric pressure DBD plasma generating device , two aspects of biomedical the application of a feasibility study . Polytetrafluoroethylene (PTFE) with a low surface free energy and chemical inertness characteristics , making it the most widely used in biomedical materials . DBD plasma method of using atmospheric pressure to improve the the polytetrafluoroethylene surface hydrophilicity , scanning electron microscopy (SEM) , by measuring the hydrophilic angle observation and X -ray photoelectron spectroscopy (XPS) analysis, the changes in the the PTFE surface properties before and after treatment the results show that , the PTFE surface oxygen atom percentage from 0 to 12.89 , hydrophilic surface angle decreased from 115 ° to 42 ° . Atmospheric pressure DBD plasma processing in vitro lung cancer cells ( A549 ) , a preliminary study of its inactivation mechanism . The study shows that the process of plasma treatment of lung cancer cells , the medium temperature is not more than 314K, and does not have an impact on cell viability , and the plasma generating high-speed particles , free radicals and ultraviolet impact on cell activity . On lung cancer cells were stained and observed the cell morphology before and after the treatment , the density of different measurement processing OD values ??before and after the change by neutral red uptake method . The selection of different types of gas discharge , summed , to stimulate the incorporation of 2.5% of the oxygen in the argon discharge more radical free electrons, the most satisfactory killing of lung cancer cells .

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CLC: > Mathematical sciences and chemical > Physics > Plasma physics > The application of plasma physics
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