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Objective: To establish a mouse haploidentical cord blood transplantation model to investigate the multi-locus mismatched cord blood transplantation of hematopoietic and immune reconstitution properties. Methods: The high-dose total body irradiation pretreatment BDF1 mice , after intravenous infusion of new students the first day of DBA / 2 mice peripheral blood , dynamic observation of recipient mice transplanted hematopoietic reconstruction and acute graft-versus- host disease (GVHD) occurrence and double staining by flow further testing by mouse peripheral blood , bone marrow , spleen, and thymus myeloid, lymphoid hematopoietic cells chronically implanted level . Results: transplanted mice white blood cells, platelets , hemoglobin , respectively, in the first 18,21,18 days rebounded significantly , in which white blood cells and platelets , respectively, in the first 29 days and 36 days to return to normal , and the hemoglobin to 50 days remained partially restored. Recipients were 90% long-term survival , and does not occur significantly GVHD. 50 days after transplantation, peripheral blood flow cytometry for mice derived hematopoietic cells of the total 88.81% of nucleated cells , containing donor-derived granulocytes 33.92%, T cells 16.83% ; bone marrow cells to granulocyte cells and B cells , accounted for 42.94% and 37.24% ; spleen tissue still remains more host T cells, T cells, 42.2% of the total ; thymus tissue T cells reached complete chimerism ; peripheral blood , bone marrow and spleen organization CD11c dendritic cells are at or close to complete chimerism . Conclusion: Single copies of newborn mice contain enough blood hematopoietic stem cells, umbilical cord blood transplants can be used as a substitute , they can rebuild haploidentical hematopoietic and immune systems of mice without causing significant GVHD, and to form a stable donor cell-based chimera . Immune organs and peripheral blood immune cells has been effectively rebuilt.
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