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Treatment of PAEs Wastewater by Immobilized Microbial Cells

Author: YanZuoZuo
Tutor: HuaRiMao;LiXueDe
School: Anhui Agricultural University
Course: Environmental Engineering
Keywords: Phthalate Immobilized microorganisms Simulated wastewater treatment
CLC: X703
Type: Master's thesis
Year: 2007
Downloads: 185
Quote: 3
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Abstract


In this study, the PAEs efficient degradation bacteria Burkhorderia P (BP) for the experimental strains, embedding its fixed processing, four phthalate esters DMP, DEP, DBP and DEHP (mainly DBP) BP immobilized bacteria for the study, the biological degradation of PAEs characteristics, to optimize the and immobilized operation conditions, select the embedding material. On this basis, several environmental factors affect the degradation of DBP immobilized BP bacteria, and the use of BP bacteria the immobilized cell processing simulated wastewater, its DBP wastewater treatment. At last PAEs wastewater quality, design a of PAEs class wastewater treatment process. The results of this study are as follows: 1, respectively, using sodium alginate and PVA-embedded the BP bacteria, with free BP bacteria immobilized microbial degradation characteristics of four kinds in PAEs (DMP, DEP, DBP and DEHP) biological control study. In the same concentration level, three biological treatment methods on the four PAEs degradation rate of DMP> DEP> DBP DEHP, microbial degradation of PAEs the rate of sodium alginate immobilized beads> PVA immobilized beads> not fixed of microorganisms; the immobilized microbial overall on PAEs degradation effect is better than free microorganisms, and wider than the free bacteria adapt to the range of pH and temperature. Two immobilized for four kinds of PAEs degradation kinetics equation are in line with a kinetic equation. 2 sodium alginate-PVA composite vector of the BP bacteria immobilized, its degradation rate of DBP activity of the immobilized cells, to determine the conditions of the immobilized operation optimization: composite carrier ratio of 8% PVA 1 % sodium alginate; saturated boric acid 2? the Cl 2 crosslinking time for 20h; immobilized cell particle diameter of 2-3mm; package bacterial amount of 3%; bacteria an amount of about 3000mg / L (wet cell weight / wastewater volume). After the mechanical strength and the matrix permeability test, indicating that the immobilized beads with good mechanical strength and the matrix permeability to embedding the composite carrier. Three pairs of pH, temperature and oxygen flow three environmental factors on sodium alginate-PVA immobilized microbial free bacteria degradation of PAEs study: sodium alginate-PVA immobilized microbial degradation of DBP were higher than the free bacteria, to adapt to the pH range, and that the immobilized cells have a certain pH adjustment capability; immobilized bacteria better adaptability to temperature change, the DBP of the removal rate at 75%, in the range of 20-40 ℃ above the optimum temperature is 30 ℃; shaking speed lower than 90r/min, the degradation rate of immobilized cells for DBP than free bacteria low and more, while the rotation speed higher than 90r/min, immobilized cells DBP degradation rate is higher than free bacteria, the shake bottle ventilation conditions degradation activity of the immobilized cells is relatively large. BP bacteria immobilized cell treatment of simulated wastewater, when the wastewater COD 3000mg / L, you can quickly remove wastewater COD. The simulated wastewater sewage preparation DBP immobilized with sodium alginate-PVA ball handling, separate control without immobilized beads. The experimental results show that the embedding BP bacteria can effectively degrade DBP, the next day on the degradation rate can reach up to 70% of natural bacteria for the DBP almost no ability to degrade. Reactor-plus-fixed bacteria for COD removal was slightly better than the control reactor, two reaction systems COD dropped to 100 mg / L, in 4-5 days.

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CLC: > Environmental science, safety science > Processing and comprehensive utilization of waste > General issues > Wastewater treatment and utilization
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