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Expressions and Changes of bcl-2 and Bax in PBMC of Allo-liver Transplantation Recipients

Author: YuHengHai
Tutor: HuMingDao;TangJiHong;HuangMing
School: Kunming Medical College
Course: Surgery
Keywords: Liver transplantation Peripheral Blood Mononuclear Cells bcl-2 bax RT-PCR
CLC: R657.3
Type: Master's thesis
Year: 2008
Downloads: 30
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Abstract


[Objective]To explore the molecular mechanism of apoptosis of Peripheral Blood Mononuclear Cells(PBMCs)and its role in the apoptosis of immune tolerance of liver transplantation,the expressions and changes of mRNA of bcl-2 and bax of PBMCs in patients suffering from allo-liver transplantation were studdied.[Methods]Cases were collected,including end-stage liver disease patients needing liver transplantation and liver transplantation recipients.Liver transplantation recipients were 10 cases from those who suffered from liver transplantation between the year of 1999 to 2007 and were administrated of FK506 post operation;end-stage liver disease patients were 10 cases from our hospital’s center of liver disease.The cases were assembled some time which were from three different groups:normal control group,end-stage liver disease control group and liver transplantation recipients group.Peripheral Blood Mononuclear Cells were separated from fresh peripheral blood by lymphocyte separation medium in the shortest time as possible in three groups.The total RNA in Peripheral Blood Mononuclear Cells were extracted and the expressions and changes of mRNA of bcl-2 and bax of Peripheral Blood Mononuclear Cells(PBMCs)in three groups were investigated by the method of RT-PCR.[Results]The mRNA of bax and bcl-2 were expressed by Peripheral Blood Mononuclear Cells(PBMCs).The level of expression of mRNA of bax was not significantly different among three groups(p=0.97,>0.05).However,the level of expression of mRNA of bcl-2 was significantly different among three groups,which of liver transplantation recipients group was significantly higher than that of normal group and end-stage liver disease group.And end-stage liver disease group’s was significantly higher than that of normal group,it has statistical significance(p=0.033,<0.05).[Conclusion]Bcl-2 and bax were ideal index for detecting apoptosis of Peripheral Blood Mononuclear Cells.There was a higher expression of bcl-2 mRNA level of Peripheral Blood Mononuclear Cells in liver transplantation recipients taking immuno-suppressive drugs,which inhibited apoptosis of Peripheral Blood Mononuclear Cells and made them have a high resistance to their apoptosis.

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CLC: > Medicine, health > Surgery > Of surgery > Abdominal surgery > Liver and liver tube
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