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Study of New Electrogenerated Chemiluminescence Method Based on Tris (2,2’-bipyridyl)Ruthenium(Ⅱ)

Author: ZhuXiaoYan
Tutor: LiangRuPing
School: Nanchang University
Course: Analytical Chemistry
Keywords: ECL Verapamil hydrochloride Ruthenium bipyridine Capillary Electrophoresis The graphene - Polydopamine - bipyridine ruthenium Polydopamine - gold nanoparticles - bipyridine ruthenium Graphene / Montmorillonite / bipyridine ruthenium ECL sensor
CLC: O627.8
Type: Master's thesis
Year: 2011
Downloads: 78
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Abstract


This thesis consists of two parts of the synthesis and study. The first part is the overview part, the second part is the part of the research report. The research report consists of four experimental system. Summary section is a brief review of the history of the development of the ECL (electrogenerated chemiluminescence, ECL) analysis; Introduction to the basic principles of the ECL reaction and the major reaction system in the ECL progress; highlight ECL analysis associated with technology as well as in drug analysis; Finally, a brief summary of the chemically modified electrode technology and nanotechnology ECL analysis. The research report mainly include the following aspects: 1 based the alkaline medium verapamil hydrochloride (VRPM) the couplet pyridine ruthenium (Ru (bpy) 32) the sensitizing effect ECL, and capillary electrophoresis technology associated capillary electrophoresis (CE) with end-column electrical the isolated ECL (CE-ECL) detection VRPM new method. Investigation Determination VRPM the optimum conditions, and successfully used for the separation and Determination of drugs and human urine VRPM. Under optimized conditions, VRPM migration time of 234.1 s, the linear range of 7.0 × 10-7 to 5.0 × 10-4 M (r = 0.9991), and a detection limit of 4.6 x 10-8M, relatively high migration time and peak The standard deviation is 1.2% and 2.08%, respectively. The same time, the article also discusses interaction between VRPM protein determination of the binding constants and maximal binding results are accurate and reliable, can be used for the analysis of drugs in clinical samples VRPM testing and quality control, and has laid for further pharmacological studies the foundation. The introduction of the the high biocompatibility self polymeric materials dopamine (DA) electroluminescent chemiluminescence sensing interface design, graphene (GO) and Ru (bpy) 32 DA self-polymerization process in situ fixed in electrode surface, the development of a simple, rapid luminescent reagent immobilization methods. The method is successful the luminous substance Ru (bpy) 32 molecules at the electrode surface high load, high activity and stability of a fixed, not only effectively prevent the leakage of Ru (bpy) 32, but also improves the Ru (bpy) 32 luminous efficiency. The sensor highly sensitive detection of tripropylamine (TPA), the linear range of 5.0 × 10-9 ~ 1.0 × 10-5 M, and a correlation coefficient of 0.9989 and a detection limit of 5 x 10-10M. The sensor continuously cyclic voltammetry lap 19, the ECL intensity of the relative standard deviation is 2.6%, showing good stability. Poly dopamine (PDA) excellent chemical stability and good biocompatibility, to show good prospects for the application of the sensor in the field of biomedical detection. 3. Prepared Polydopamine - gold nanoparticles-Ru (bpy) 32 (PDA-AuNPs-Ru (bpy) 32) composite film modified glassy carbon electrode prepared a new Ru (bpy) 32 electric ECL sensor. Inspected the sensor TPA as co-reactant in PBS electrochemical behavior and ECL acts. The linear range of the sensor to measure the TPA to 5.0 × 10-8-6.0 × 10-5M, a correlation coefficient of 0.9997, the detection limit of 1 × 10 8 M PDA-AuNPs-Ru (bpy) 32 / GCE cyclic voltammetry security scan 28 laps of the electrical induced chemiluminescence signal more stable, and the relative standard deviation of 1.9% and the modified electrode placed in the air and 0.1 M pH 7.8 PBS for 30 days, the ECL activity were 77% and 89%, indicating that the sensor has excellent stability. 4 based montmorillonite (Montmorillonite, MMT) good adsorption, ion exchange capacity, excellent adhesion, stability, combined with the surface of the graphene large number of negatively charged groups, and is both a large specific surface area, prepared graphene / Montmorillonite / Ru (bpy) 32 (GO / MMT / Ru (bpy) 32) nanocomposite modified glassy carbon electrode prepared a new Ru (bpy) 32 electric ECL sensor. effects of TPA as a co-reactant in PBS, the sensor electrochemical behavior and ECL behavior GO excellent conductive properties can promote electrons in the electrode surface pass, and the GO and the MMT a large specific surface area and the lamellar structure of the GO / MMT / Ru (bpy) 32 composite film having a more open structure and a larger surface area, thereby allowing Ru (bpy) 32 in the film faster diffusion, use of the composite, a large specific surface area and MMT excellent cation exchange properties effectively increase the immobilization amount of the Ru (bpy) 32, and the GO introduced to prevent the Ru (bpy) 32 to a membrane electrochemical inert hydrophobic diffusion of the sensor for the detection of TPA, the linear range of 5.0 × 10-8-6.0 × 10-5M, the correlation coefficient is 0.9988, the detection limit is 5.0 × 10-9M. continuous cyclic voltammetry the sensor Scan 23 laps the ECL intensity relative standard deviation of 3.7%. The modified electrode was placed in the air and 100mM pH 8.0 PBS for 30 days, its ECL activity of 76% and 81%, respectively, indicating that the sensor has excellent stability of MMT excellent stability, good adsorption properties and GO excellent conductive properties of the sensor will show a good prospect in the field of drug testing.

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CLC: > Mathematical sciences and chemical > Chemistry > Organic Chemistry > Elements in organic compounds > Ⅷ of group elements, organic compounds
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