Dissertation > Excellent graduate degree dissertation topics show

Praparation of Immunomagnefic Nanoparficles and Its Application in the Separation of CD34~+ Cell

Author: ZhaoLiNa
Tutor: ShenHeBai
School: Shanghai Normal University
Course: Physical and chemical
Keywords: Magnetic nanoparticles Nitrobenzaldehyde colorimetry Amino CD34 ~ cells CD34 antigen Hematopoietic stem / progenitor cells Umbilical Cord Blood
CLC: TB383.1
Type: Master's thesis
Year: 2008
Downloads: 141
Quote: 1
Read: Download Dissertation

Abstract


Since nanotechnology generate nanotechnology has been widespread concern and rapid development which magnetic nanoparticles because of its uniform particle size, large specific surface area, the superparamagnetic advantages, favored by researchers in protein and immobilized enzyme immunoassays, RNA and DNA purification, cell separation and identification, targeting, play an increasingly important role in the administration and PCR. Hematopoietic stem cell transplantation to treat leukemia, lymphoma, multiple myeloma and other malignant diseases of the blood system, has an important significance in medicine. However a prerequisite step separating the high purity of the hematopoietic stem cells are hematopoietic stem cell transplantation, become the limiting factors for hematopoietic cell engineering. The hematopoietic stem cells have a variety of cell surface markers, but the identification of hematopoietic stem cells are the most commonly used is the CD34 antigen. CD34 monoclonal antibody may be the identification of specific hematopoietic stem cell surface CD34 antigen, therefore the use of the magnetic nanoparticles with CD34 monoclonal antibody binding forming a magnetic nanoparticle - antibody complexes the immune magnetic nanoparticles, the rapid separation of a high applied magnetic field The purity of the CD34 cells. This paper studies the immune Magnetic Nanoparticles and CD34 cells were isolated from umbilical cord blood. (1) with superparamagnetic γ-Fe2O3 nanoparticles synthesized by co-precipitation method, by TritonX-100, n-hexanol, cyclohexane-posed inverse microemulsion system, the TEOS ammonia catalytic The hydrolysis of the surface of the γ-Fe2O3 wrapped layer having a biological affinity for the silica layer to form a core-shell type magnetic nanoparticles. Having active amino functional group on its surface modified with aminosilane. TEM showed that the magnetic nanoparticles was a uniform particle size and good monodispersibility; the Infrared Characterization amino has been successfully modified at the surface of the magnetic nanoparticles. Aminated magnetic nanoparticles by a chemical reaction with antibodies, the formation of magnetic nanoparticle - antibody complexes, and through the UV characterization. Magnetic nanoparticles with different antibodies connection can be separated from the different cells, and provide a means for the separation of biological cells. (2), nitrobenzaldehyde colorimetric assay determination of amino quick and easy, and non-toxic, has a lot of advantages compared with other methods. Adoption of nitrobenzaldehyde nanometer magnetic particle interface amino quantitative colorimetric assay of the subsequent quantitative connection biological molecules is very important; nitrobenzaldehyde colorimetric assay also help to study the magnetic nanoparticles under different reaction conditions The amount of surface interface amino reactive silane interface helps to optimize and need further study the influence of the reaction conditions in the production of high-performance materials. (3) magnetic nanoparticles-CD34 antibody complex was added to the need to separate the samples, magnetic nanoparticles can be carried out with the target cell-specific binding, and then the external magnetic field on the attached the magnetic nanoparticles target cell retention. thereby achieve separation. The results showed the use of magnetic nanoparticles - antibody complex can be quickly and efficiently CD34 ~ cells from umbilical cord blood the grab elected separated CD34 ~ cells remain normal morphology, a high proliferative capacity and differentiation ability, indicating magnetic nanoparticle - antibody complex was separated CD34 ~ cell technology has a good effect.

Related Dissertations

  1. Synthesis and Quality Control Research of ATPR,TQ463
  2. Traumatic Brain Injury Patients with White Blood Cells in Peripheral Blood CD34~+ Cells and Content Changes,R651.1
  3. Complex functions drug-loaded microspheres and in spinal cord injury treatment Application of,R943
  4. Cyclodextrin -modified magnetic nanoparticles synthesis and study drug carrier,TQ460.1
  5. Studies on Magnetic Nanocomposite Particles as Drug Carriers for Photodynamic Therapy Agents,TB383.1
  6. Construction of Multi-responsive Gene Vector Via Layer-by-Layer Assembly,TB383.1
  7. A non- organic solid particles in a silver paste and process performance properties of,TN604
  8. Nano- phenyl silicone resin preparation and application,TB383.1
  9. The Study of Preparation Electrochemical Properties and Surface Modification of Boron-doped Diamond Thin Film,TB383.2
  10. Synthesis and Characterization on Semi-Aromatic Polyamides Containing Benzoxazole Units,O633.5
  11. The Effect of KanXianWan(KXW) in the Pentylenetetrazol Model of the Chronic Epileptic Rats,R285.5
  12. Mechanical polishing γ- aminobutyric acid synthesis precursors and the condensation reaction of Applied Research,R914
  13. Rare on crop body protein , amino acids and effector impact study,S312
  14. Peony blossom and aroma substances in the aging process and the related changes in enzyme activity,S685.11
  15. Effect of Grafting on Biosynthesis of Esters Through Amino Acid Pathway in Oriental Sweet Melon,S652
  16. Research of Ireland-Claisen Rearrangement of Butenyl Trifluoroacetate and Asymmetric Syntheses of α-fluoro-β-amino Acids,O621.34
  17. E-waste contaminated intrauterine exposure to polybrominated diphenyl ethers fetal growth and placental IGF-1 and IGFBP-3 expression,R114
  18. Study on Extraction, Purification and Activity of Polysaccharide in Saccharum Alhagi,R284.1
  19. Physiological State and Genetic Stability of Cord Blood Cells during Ex Vivo Expansion,R329
  20. Biological Mass Spectrometry techniques and methods in protein drugs Sequence Identification Application Research,O657.63
  21. Studies on Effective Composition and Liver Protective Action of Polysaccharides from Different Parts of the Artificial Cultivation Cordyceps,R285

CLC: > Industrial Technology > General industrial technology > Materials science and engineering > Special structural materials
© 2012 www.DissertationTopic.Net  Mobile